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Plasmid construction

Novel and effective plasmid transfection protocols for functional analysis of genetic elements in human cardiac fibroblasts

Makoto Matsuyama, Takahiro Iwamiya

published 26 Nov 2024

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A cryptic promoter in the exon of HKR1 drives expression of a truncated form of Hkr1 in Saccharomyces cerevisiae

Toshihiro Kondo, Yuna Hosokawa, Ryotaro Ozawa, Shin Kasahara

published 21 Nov 2024

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The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

Sujit Jung Karki, Paola Pilo, Colleen Lawless, Nikolaos Mastrodimos, Anna M. M. Tiley, James Burke, Angela Feechan

published 19 Nov 2024

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Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

published 14 Nov 2024

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Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

Conghui Zhang, Qingfeng Ma, Wei Wang, Hui Song, Xue Wang, Fengxia Xu, Chengliang Zhu, Xinghui Liu

published 12 Nov 2024

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In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system

Ryota Tanaka, Kenji Tamao, Mana Ono, Seiya Yamayoshi, Yoshihiro Kawaoka, Masayuki Su’etsugu, Hiroyuki Noji, Kazuhito V. Tabata

published 08 Nov 2024

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Dolichol kinases from yeast, nematode and human can replace each other and exchange their domains creating active chimeric enzymes in yeast

Danguole Ziogiene, Andrius Burdulis, Albertas Timinskas, Ruta Zinkeviciute, Emilija Vasiliunaite, Milda Norkiene, Alma Gedvilaite

published 07 Nov 2024

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The N-terminal activation function AF-1 domain of ERα interacts directly with the C-terminal AF-2-holding ligand-binding domain to recruit the coactivator proteins

Xiaohui Liu, Yutaka Matsuyama, Makiko Sugiyama, Keitaro Suyama, Takeru Nose, Miki Shimohigashi, Yasuyuki Shimohigashi

published 21 Oct 2024

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A modified CD9 tag for efficient protein delivery via extracellular vesicles

Shojiro Inano, Toshiyuki Kitano

published 17 Oct 2024

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Analysis of low-density lipoprotein receptor gene mutations in a family with familial hypercholesterolemia

Ya-nan Hu, Min Wu, Hong-ping Yu, Qiu-yan Wu, Ying Chen, Jian-Hui Zhang, Dan-dan Ruan, Yan-ping Zhang, Jing Zou, Li Zhang, Xin-fu Lin, Zhu-ting Fang, Li-Sheng Liao, Fan Lin, Hong Li, Jie-Wei Luo

published 11 Oct 2024

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MultiGreen: A multiplexing architecture for GreenGate cloning

Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott

published 18 Sep 2024

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Transcriptional control of a stem cell factor nucleostemin in liver regeneration and aging

Xiaoqin Liu, Junying Wang, Fang Li, Nikolai Timchenko, Robert Y. L. Tsai

published 11 Sep 2024

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Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenic Phytophthora species

Erika N. Dort, Richard C. Hamelin

published 10 Sep 2024

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