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Plasmid construction

Novel and effective plasmid transfection protocols for functional analysis of genetic elements in human cardiac fibroblasts

Makoto Matsuyama, Takahiro Iwamiya

A cryptic promoter in the exon of HKR1 drives expression of a truncated form of Hkr1 in Saccharomyces cerevisiae

Toshihiro Kondo, Yuna Hosokawa, Ryotaro Ozawa, Shin Kasahara

The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

Sujit Jung Karki, Paola Pilo, [ ... ], Angela Feechan

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

Conghui Zhang, Qingfeng Ma, [ ... ], Xinghui Liu

In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system

Ryota Tanaka, Kenji Tamao, [ ... ], Kazuhito V. Tabata

Dolichol kinases from yeast, nematode and human can replace each other and exchange their domains creating active chimeric enzymes in yeast

Danguole Ziogiene, Andrius Burdulis, [ ... ], Alma Gedvilaite

The N-terminal activation function AF-1 domain of ERα interacts directly with the C-terminal AF-2-holding ligand-binding domain to recruit the coactivator proteins

Xiaohui Liu, Yutaka Matsuyama, [ ... ], Yasuyuki Shimohigashi

A modified CD9 tag for efficient protein delivery via extracellular vesicles

Shojiro Inano, Toshiyuki Kitano

Analysis of low-density lipoprotein receptor gene mutations in a family with familial hypercholesterolemia

Ya-nan Hu, Min Wu, [ ... ], Jie-Wei Luo

MultiGreen: A multiplexing architecture for GreenGate cloning

Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott

Transcriptional control of a stem cell factor nucleostemin in liver regeneration and aging

Xiaoqin Liu, Junying Wang, [ ... ], Robert Y. L. Tsai

Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenic Phytophthora species

Erika N. Dort, Richard C. Hamelin

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