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Plasmid construction

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

Synthesis of Morphinan Alkaloids in Saccharomyces cerevisiae

Elena Fossati, Lauren Narcross, [ ... ], Vincent J. J. Martin

Large Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9

Luqing Zhang, Ruirui Jia, [ ... ], Yaowu Zheng

Assembly of Designer TAL Effectors by Golden Gate Cloning

Ernst Weber, Ramona Gruetzner, [ ... ], Sylvestre Marillonnet

mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities

Ann L. McEvoy, Hiofan Hoi, [ ... ], Robert E. Campbell

A Simple and Sensitive Method for Measuring Tumor-Specific T Cell Cytotoxicity

Xinping Fu, Lihua Tao, [ ... ], Xiaoliu Zhang

Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen

Tyler W. McCullock, David M. MacLean, Paul J. Kammermeier

Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Song Yang, Ye Kuang, [ ... ], Donghai Wu

Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications

Yafeng Song, Jonas M. Nikoloff, [ ... ], Dawei Zhang

Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells

Franziska Stagge, Gyuzel Y. Mitronova, [ ... ], Stefan Jakobs

Plasmid Metagenome Reveals High Levels of Antibiotic Resistance Genes and Mobile Genetic Elements in Activated Sludge

Tong Zhang, Xu-Xiang Zhang, Lin Ye

Green Fluorescent Protein Labeling of Listeria, Salmonella, and Escherichia coli O157:H7 for Safety-Related Studies

Li Ma, Guodong Zhang, Michael P. Doyle

Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

Alexander Ulrich, Kasper R. Andersen, Thomas U. Schwartz

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