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Plasmid construction

A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters

Yun-Cheol Kim, Sherie L. Morrison

Cell-to-Cell Transformation in Escherichia coli: A Novel Type of Natural Transformation Involving Cell-Derived DNA and a Putative Promoting Pheromone

Rika Etchuuya, Miki Ito, [ ... ], Sumio Maeda

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

Manuel Stemmer, Thomas Thumberger, [ ... ], Juan L. Mateo

A Robust High Throughput Platform to Generate Functional Recombinant Monoclonal Antibodies Using Rabbit B Cells from Peripheral Blood

Stefan Seeber, Francesca Ros, [ ... ], Sonja Offner

A Practical Comparison of Ligation-Independent Cloning Techniques

Julian Stevenson, James R. Krycer, Lisa Phan, Andrew J. Brown

Serious Overestimation in Quantitative PCR by Circular (Supercoiled) Plasmid Standard: Microalgal pcna as the Model Gene

Yubo Hou, Huan Zhang, Lilibeth Miranda, Senjie Lin

A Comparison of Exogenous Promoter Activity at the ROSA26 Locus Using a PhiC31 Integrase Mediated Cassette Exchange Approach in Mouse ES Cells

Chiann-mun Chen, Jon Krohn, Shoumo Bhattacharya, Benjamin Davies

Inverse Fusion PCR Cloning

Markus Spiliotis

A New and Improved Host-Independent Plasmid System for RK2-Based Conjugal Transfer

Trine Aakvik Strand, Rahmi Lale, [ ... ], Svein Valla

Plasmid Construction Using Recombination Activity in the Fission Yeast Schizosaccharomyces pombe

Ayako Chino, Kenji Watanabe, Hisao Moriya

A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

Andreas Binder, Jayne Lambert, [ ... ], Martin Parniske

Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

Chih-Hui Lin, Yu-Chieh Chen, Tzu-Ming Pan

Improved Genome Editing in Human Cell Lines Using the CRISPR Method

Ivan M. Munoz, Piotr Szyniarowski, [ ... ], Christophe Lachaud

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