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Plasmid construction

A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters

Yun-Cheol Kim, Sherie L. Morrison

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

Manuel Stemmer, Thomas Thumberger, [ ... ], Juan L. Mateo

Cell-to-Cell Transformation in Escherichia coli: A Novel Type of Natural Transformation Involving Cell-Derived DNA and a Putative Promoting Pheromone

Rika Etchuuya, Miki Ito, [ ... ], Sumio Maeda

A Robust High Throughput Platform to Generate Functional Recombinant Monoclonal Antibodies Using Rabbit B Cells from Peripheral Blood

Stefan Seeber, Francesca Ros, [ ... ], Sonja Offner

A Practical Comparison of Ligation-Independent Cloning Techniques

Julian Stevenson, James R. Krycer, Lisa Phan, Andrew J. Brown

Serious Overestimation in Quantitative PCR by Circular (Supercoiled) Plasmid Standard: Microalgal pcna as the Model Gene

Yubo Hou, Huan Zhang, Lilibeth Miranda, Senjie Lin

A Comparison of Exogenous Promoter Activity at the ROSA26 Locus Using a PhiC31 Integrase Mediated Cassette Exchange Approach in Mouse ES Cells

Chiann-mun Chen, Jon Krohn, Shoumo Bhattacharya, Benjamin Davies

Inverse Fusion PCR Cloning

Markus Spiliotis

A New and Improved Host-Independent Plasmid System for RK2-Based Conjugal Transfer

Trine Aakvik Strand, Rahmi Lale, [ ... ], Svein Valla

A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

Andreas Binder, Jayne Lambert, [ ... ], Martin Parniske

Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

Chih-Hui Lin, Yu-Chieh Chen, Tzu-Ming Pan

Plasmid Construction Using Recombination Activity in the Fission Yeast Schizosaccharomyces pombe

Ayako Chino, Kenji Watanabe, Hisao Moriya

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

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