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Plasmid construction

The rsmA mutant from Pseudomonas aeruginosa ID4365 is a non-virulent strain that is suitable for pyocyanin and phenazine-1-carboxylic acid production

Misael Josafat Fabian Del-Olmo, Luis Fernando Montelongo-Martínez, Gisela Viridiana Villafañe-Barajas, Abigail González-Valdez, Veronica Roxana Flores-Vega, Roberto Rosales-Reyes, Marco Elias Gudiño-Zayas, Thelma Rizo-Pica, Raunel Tinoco-Valencia, Adelfo Escalante, Gloria Soberón-Chávez, Miguel Cocotl-Yañez

published 04 Dec 2025

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Disrupting ribulose-5-phosphate metabolic flux enhances riboflavin production in Escherichia coli BL21(DE3)

Junhui Ying, Jin Lu, Qiming Liu, Yingjie Pan, Xianfeng Bao, Junjun Yin, Bing Fu

published 14 Nov 2025

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Toward an all-in-one recombinant adeno-associated virus vector for functionally ablating the prion gene using CRISPR-Cas technology

Claire Verkuyl, Ari Belotserkovsky, Thomas Zerbes, Declan Williams, Medha R. Krishnan, Sabrina Zhu, Sophie Grunnesjӧ, Shehab Eid, Cunjie Zhang, Wenda Zhao, Leo Xu, Eleanore Lin, Teaghan O’Shea, Benjamin Draper, Andreas Jungman, Patrick Most, Gerold Schmitt-Ulms

published 07 Nov 2025

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One IV HEDGES DNA vector administration encoding hGLA or hGH produces durable hGLA and hGH serum lvels in immunocompetent mice

Alice Ye, Marissa Mack, Sarah Ursu, Stephen Chmura, Robert Steiner, Tim Heath, Chakkrapong Handumrongkul, Robert Debs

published 31 Oct 2025

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One SQ HEDGES DNA vector only dose produces durable hGLA or anti-SARS-CoV-2 mAb therapeutic serum protein levels

Alice Ye, Marissa Mack, Ryan Ice, Tim Heath, Elof Ericksson, Stanford Shoor, Robert D. Steiner, Chakkrapong Handumrongkul, Robert Debs

published 31 Oct 2025

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Functional expression of a Mo-dependent formate dehydrogenase in Escherichia coli under aerobic conditions

Marion Schulz, Anne Berger, David Roche, Emilie Pateau, Ivan Dubois, Valérie A. Delmas, Mélodie Cadillon, Madeleine Bouzon, Volker Döring

published 29 Oct 2025

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Evaluating the effects of synthetic POM cycles and NAD⁺ kinase expression on fatty alcohol production in Saccharomyces cerevisiae

Bonnie A. McNeil, Charfeddine Khalifa, Anagha Krishnan, David T. Stuart

published 29 Sep 2025

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The GravyTrain toolbox for molecular cell biology

Oren Shatz, Zvulun Elazar

published 17 Sep 2025

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Glutamate dehydrogenase from Pantoea ananatis: A new bacterial enzyme with dual coenzyme specificity

Maria S. Kharchenko, Victoria S. Skripnikova, Julia G. Rostova, Natalia P. Zakataeva

published 19 Aug 2025

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Establishing a non-viral transfection system for fibroblasts using branched polyethylenimine

Tim Lukas Lübbersmeyer, Kirsten Wissel, Andrea Hoffmann, Lisa Kötter, Thomas Lenarz, Gerrit Paasche

published 05 Aug 2025

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scFv intrabody targeting wildtype TDP-43 presents protective effects in a cellular model of TDP-43 proteinopathy

Yara Al Ojaimi, Rudolf Hergesheimer, Anna A. Chami, Hugo Alarcan, Johanna Augros, Audrey Dangoumau, Shanez Haouari, Jérôme Bourgeais, Antoine Lefevre, Samira Osman, Patrick Emond, Patrick Vourc’h, Christian R. Andres, Philippe Corcia, Olivier Herault, Pierre Martineau, Débora Lanznaster, Hélène Blasco

published 04 Aug 2025

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MTMR7 regulates human spermatogonial stem cells proliferation and migration via targeting FLNB

Nianchao Zhou, Tiantian Wu, Yi Yu, Wenxin Gao, Bing Jiang, Haoyue Hu, Xiaoyan Huang, Cong Shen, Yibo Wu, Tingting Gao

published 10 Jul 2025

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pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2

Inês M. Luís, Mariana Parada, João B. Vicente, Isabel A. Abreu

published 07 Jul 2025

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