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Plasmid construction

One SQ HEDGES DNA vector only dose produces durable hGLA or anti-SARS-CoV-2 mAb therapeutic serum protein levels

Alice Ye, Marissa Mack, Ryan Ice, Tim Heath, Elof Ericksson, Stanford Shoor, Robert D. Steiner, Chakkrapong Handumrongkul, Robert Debs

published 31 Oct 2025

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Functional expression of a Mo-dependent formate dehydrogenase in Escherichia coli under aerobic conditions

Marion Schulz, Anne Berger, David Roche, Emilie Pateau, Ivan Dubois, Valérie A. Delmas, Mélodie Cadillon, Madeleine Bouzon, Volker Döring

published 29 Oct 2025

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Evaluating the effects of synthetic POM cycles and NAD⁺ kinase expression on fatty alcohol production in Saccharomyces cerevisiae

Bonnie A. McNeil, Charfeddine Khalifa, Anagha Krishnan, David T. Stuart

published 29 Sep 2025

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The GravyTrain toolbox for molecular cell biology

Oren Shatz, Zvulun Elazar

published 17 Sep 2025

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Glutamate dehydrogenase from Pantoea ananatis: A new bacterial enzyme with dual coenzyme specificity

Maria S. Kharchenko, Victoria S. Skripnikova, Julia G. Rostova, Natalia P. Zakataeva

published 19 Aug 2025

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Establishing a non-viral transfection system for fibroblasts using branched polyethylenimine

Tim Lukas Lübbersmeyer, Kirsten Wissel, Andrea Hoffmann, Lisa Kötter, Thomas Lenarz, Gerrit Paasche

published 05 Aug 2025

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scFv intrabody targeting wildtype TDP-43 presents protective effects in a cellular model of TDP-43 proteinopathy

Yara Al Ojaimi, Rudolf Hergesheimer, Anna A. Chami, Hugo Alarcan, Johanna Augros, Audrey Dangoumau, Shanez Haouari, Jérôme Bourgeais, Antoine Lefevre, Samira Osman, Patrick Emond, Patrick Vourc’h, Christian R. Andres, Philippe Corcia, Olivier Herault, Pierre Martineau, Débora Lanznaster, Hélène Blasco

published 04 Aug 2025

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MTMR7 regulates human spermatogonial stem cells proliferation and migration via targeting FLNB

Nianchao Zhou, Tiantian Wu, Yi Yu, Wenxin Gao, Bing Jiang, Haoyue Hu, Xiaoyan Huang, Cong Shen, Yibo Wu, Tingting Gao

published 10 Jul 2025

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pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2

Inês M. Luís, Mariana Parada, João B. Vicente, Isabel A. Abreu

published 07 Jul 2025

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Coli bond: A dual-function encryption system for secure information storage and transmission by microorganisms

Xuefeng Xiao, Yunuo Song, Jingxuan Hu, Jinchen Han, Wanbin Xing, Chang Liu

published 11 Jun 2025

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Success and limitations in adaptation of Fast-TrACC tissue culture-independent transformation in coffee, cotton, and tree tobacco

Boaz Negin, Laura Daniela Martin-Lesmes, Georg Jander

published 15 May 2025

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Promiscuous structural cross-compatibilities between major shell components of Klebsiella pneumoniae bacterial microcompartments

Lucie Barthe, Damien Balestrino, Bessam Azizi, Delphine Dessaux, Vanessa Soldan, Jeremy Esque, Thomas Schiex, Sophie Barbe, Luis F. Garcia-Alles

published 07 May 2025

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Correlation of p53 oligomeric status and its subcellular localization in the presence of the AML-associated NPM mutant

Aleš Holoubek, Dita Strachotová, Kateřina Wolfová, Petra Otevřelova, Sára Belejová, Pavla Röselová, Aleš Benda, Barbora Brodská, Petr Herman

published 07 May 2025

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