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Plasmid construction

Manipulating the type VI secretion system spike to shuttle passenger proteins

Sarah Wettstadt, Alain Filloux

An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

Jiankang Jin, Yan Xu, [ ... ], Jaffer A. Ajani

AMPKα Subunit Ssp2 and Glycogen Synthase Kinases Gsk3/Gsk31 are involved in regulation of sterol regulatory element-binding protein (SREBP) activity in fission yeast

Hao Miao, Qiannan Liu, [ ... ], Yue Fang

Hydrodynamics-based liver transfection achieves gene silencing of CB1 using short hairpin RNA plasmid in cirrhotic rats

Adriana Díaz-Rivera, Alejandra Meza-Ríos, [ ... ], Ana Sandoval-Rodríguez

Very rapid cloning, expression and identifying specificity of T-cell receptors for T-cell engineering

Shan Zong, Tiejuan Mi, [ ... ], Hiroki Torikai

Improved cell-specificity of adeno-associated viral vectors for medullary thyroid carcinoma using calcitonin gene regulatory elements

Hazel C. Levy, Danielle Hulvey, [ ... ], Jacqueline A. Hobbs

Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen

Tyler W. McCullock, David M. MacLean, Paul J. Kammermeier

Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing

Chang-ye Hui, Yan Guo, [ ... ], Wen Zhang

Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus

Ryan Guillemette, Blake Ushijima, [ ... ], Farooq Azam

Gene expression noise in a complex artificial toxin expression system

Alexandra Goetz, Andreas Mader, [ ... ], Madeleine Opitz

Liganded T3 receptor β2 inhibits the positive feedback autoregulation of the gene for GATA2, a transcription factor critical for thyrotropin production

Naoko Hirahara, Hiroko Misawa Nakamura, [ ... ], Takafumi Suda

Potentiation of curing by a broad-host-range self-transmissible vector for displacing resistance plasmids to tackle AMR

Alessandro Lazdins, Anand Prakash Maurya, [ ... ], Christopher M. Thomas

Repository-based plasmid design

Joshua J. Timmons, Doug Densmore

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