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Plasmid construction

Knockout of Babesia bovis rad51 ortholog and its complementation by expression from the BbACc3 artificial chromosome platform

Erin A. Mack, Yu-Ping Xiao, David R. Allred

Strategies found not to be suitable for stabilizing high steroid hydroxylation activities of CYP450 BM3-based whole-cell biocatalysts

Carolin Bertelmann, Bruno Bühler

Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation

Dana Kolibachuk, Benjamin J. Ryder, [ ... ], Ashley Nieves

Enhanced hypocrellin production of Shiraia sp. SUPER-H168 by overexpression of alpha-amylase gene

Ruijie Gao, Zhecun Xu, [ ... ], Yujie Cai

LupiQuant: A real-time PCR based assay for determining host-to-parasite DNA ratios of Onchocerca lupi and host Canis lupus from onchocercosis samples

Chandler C. Roe, Jennifer Urbanz, [ ... ], Jason W. Sahl

Cas9 is mostly orthogonal to human systems of DNA break sensing and repair

Ekaterina A. Maltseva, Inna A. Vasil’eva, [ ... ], Olga I. Lavrik

Development and validation of monoclonal antibodies specific for Candida albicans Als2, Als9-1, and Als9-2

Soon-Hwan Oh, David A. Coleman, Xiaomin Zhao, Lois L. Hoyer

ActVI-ORFA directs metabolic flux towards actinorhodin by preventing intermediate degradation

Xuechen Zhu, Rongbin Wang, [ ... ], Mikko Metsä-Ketelä

A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction

Junwei Pan, Ming Liu, Xiaozong Duan, Dan Wang

Toward an all-in-one recombinant adeno-associated virus vector for functionally ablating the prion gene using CRISPR-Cas technology

Claire Verkuyl, Ari Belotserkovsky, [ ... ], Gerold Schmitt-Ulms

Identifying widespread and recurrent variants of genetic parts to improve annotation of engineered DNA sequences

Matthew J. McGuffie, Jeffrey E. Barrick

The N-terminal activation function AF-1 domain of ERα interacts directly with the C-terminal AF-2-holding ligand-binding domain to recruit the coactivator proteins

Xiaohui Liu, Yutaka Matsuyama, [ ... ], Yasuyuki Shimohigashi

A novel replication initiation region encoded in a widespread Acinetobacter plasmid lineage carrying a blaNDM-1 gene

Elena Bello-López, Ángeles Pérez-Oseguera, Walter Santos, Miguel Ángel Cevallos

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