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Plasmid construction

High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su

published 09 Sep 2022

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An evaluation of fusion partner proteins for paratransgenesis in Asaia bogorensis

Christina Grogan, Marissa Bennett, David J. Lampe

published 01 Sep 2022

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OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias

Artis Linars, Ivars Silamikelis, Dita Gudra, Ance Roga, Davids Fridmanis

published 08 Aug 2022

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Phosphopantetheinyl transferase ClbA contributes to the virulence of avian pathogenic Escherichia coli in meningitis infection of mice

Xia Meng, Yanfei Chen, Peili Wang, Pengpeng Xia, Jinqiu Wang, Mengping He, Chunhong Zhu, Heng Wang, Guoqiang Zhu

published 28 Jul 2022

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Sequence analysis and plasmid mobilization of a 6.6-kb kanamycin resistance plasmid, pSNC3-Kan, from a Salmonella enterica serotype Newport isolate

Chin-Yi Chen, Ly-Huong T. Nguyen, Terence P. Strobaugh Jr.

published 14 Jul 2022

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Development and validation of monoclonal antibodies specific for Candida albicans Als2, Als9-1, and Als9-2

Soon-Hwan Oh, David A. Coleman, Xiaomin Zhao, Lois L. Hoyer

published 08 Jul 2022

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A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors

Koollawat Chupradit, Nontaphat Thongsin, Chatchai Tayapiwatana, Methichit Wattanapanitch

published 07 Jul 2022

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Bombyx mori Vps13d is a key gene affecting silk yield

Luochao Zhao, Xia Sun, Xueyang Wang, Sheng Qin, Yunhui Kong, Muwang Li

published 07 Jul 2022

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Comparative analysis of dCas9-VP64 variants and multiplexed guide RNAs mediating CRISPR activation

Kohei Omachi, Jeffrey H. Miner

published 28 Jun 2022

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Evaluation of transgenic chickpea harboring codon-modified Vip3Aa against gram pod borer (Helicoverpa armigera H.)

Prateek Singh, Sujayanand G. K., Shallu Thakur, Meenal Rathore, Om Prakash Verma, Narendra Pratap Singh, Alok Das

published 24 Jun 2022

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Identification of HCN1 as a 14-3-3 client

Colten Lankford, Jon Houtman, Sheila A. Baker

published 09 Jun 2022

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Structural and biophysical characterization of the multidomain xylanase Xyl

Valentine Anye, Robert F. Kruger, Wolf-Dieter Schubert

published 03 Jun 2022

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The SARS-CoV-2 main protease doesn’t induce cell death in human cells in vitro

Alexey Komissarov, Maria Karaseva, Marina Roschina, Sergey Kostrov, Ilya Demidyuk

published 24 May 2022

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