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Nucleotide sequencing

Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

Pradip Kumar Singh, Michael S. Donnenberg

published 13 Mar 2025

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Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency

Lili Zhai, Zi Wang, Fen Liu, Chongjun Xu, Jingjing Wang, Hongyan Han, Qingqing Xie, Wenwei Zhang, Yue Zheng, Alexander K. Buell, Yuliang Dong

published 14 Feb 2025

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A new method for the reproducible development of aptamers (Neomers)

Cathal Meehan, Erika L. Hamilton, Chloé G. Mansour, Soizic Lecocq, Cole J. Drake, Yisi An, Emily Rodrigues, Gregory Penner

published 12 Feb 2025

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Whole-genome sequence analysis of SFTS bunyavirus in Huzhou, China

Deshun Xu, Lei Ji, Xiaofang Wu, Liping Chen

published 11 Feb 2025

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AutoPVPrimer: A comprehensive AI-Enhanced pipeline for efficient plant virus primer design and assessment

Abozar Ghorbani, Mahsa Rostami, Elham Ashrafi-Dehkordi, Pietro Hiram Guzzi

published 30 Jan 2025

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Genetic tracking of Crimean-Congo haemorrhagic orthonairovirus in Hyalomma population infesting cattle in Nigeria

Oluwafemi Babatunde Daodu, Joseph Ojonugwa Shaibu, Rosemary Ajuma Audu, Daniel Oladimeji Oluwayelu

published 24 Jan 2025

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Tetracycline and chloramphenicol exposure induce decreased susceptibility to tigecycline and genetic alterations in AcrAB-TolC efflux pump regulators in Escherichia coli and Klebsiella pneumoniae

Nian Anwar Nasralddin, Mehri Haeili, Sasan Karimzadeh, Fatemeh Alsahlani

published 22 Jan 2025

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Deciphering mixed infections by plant RNA virus and reconstructing complete genomes simultaneously present within-host

Martine Bangratz, Aurore Comte, Estelle Billard, Abdoul Kader Guigma, Guillaume Gandolfi, Abalo Itolou Kassankogno, Drissa Sérémé, Nils Poulicard, Charlotte Tollenaere

published 14 Jan 2025

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Detection of SARS-CoV-2 and a possible variant in shelter cats

Ogi Okwumabua, Nancy Bradley-Siemens, Catherine Cruz, Lauren Chittick, Melissa Thompson

published 13 Jan 2025

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Plasmodium falciparum surf_4.1 in clinical isolates: From genetic variation and variant diversity to in silico design immunopeptides for vaccine development

Nitchakarn Noranate, Jariya Sripanomphong, Fingani Annie Mphande- Nyasulu, Suwanna Chaorattanakawee

published 30 Dec 2024

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Assessment of salivary microRNA by RT-qPCR: Facing challenges in data interpretation for clinical diagnosis

Marc Van Der Hofstadt, Anna Cardinal, Morgane Lepeltier, Jérémy Boulestreau, Alimata Ouedraogo, Malik Kahli, Pierre Champigneux, Laurence Molina, Franck Molina, Thi Nhu Ngoc Van

published 10 Dec 2024

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Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses

Kouichi Kitamura, Minetaro Arita

published 10 Dec 2024

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stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing

Jason D. Limberis, Roland J. Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell, Alina Nalyvayko, Zach Howard, Grant Theron, Rouxjeane Venter, John Z. Metcalfe

published 09 Dec 2024

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