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Nested polymerase chain reaction

A Borrelia burgdorferi outer surface protein C (OspC) genotyping method using Luminex technology

Patrick Pearson, Olivia Skaltsis, Chu-Yuan Luo, Guang Xu, Zachary Oppler, Dustin Brisson, Stephen M. Rich

published 01 Jun 2022

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Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus

Woong Sik Jang, Da Hye Lim, Young Lan Choe, Jeonghun Nam, Kyung Chul Moon, Chaewon Kim, Minkyeong Choi, Insu Park, Dae Won Park, Chae Seung Lim

published 16 Feb 2022

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Rapid molecular diagnosis of Parechovirus infection using the reverse transcription loop-mediated isothermal amplification technique

Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada

published 29 Nov 2021

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Nested PCR followed by NGS: Validation and application for HPV genotyping of Tunisian cervical samples

Monia Ardhaoui, Emna Ennaifer, Anna Christina De Matos Salim, Flávio Marcom Gomez, Thalja Laasili, Med Samir Boubaker, Ikram Guizani

published 11 Aug 2021

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Field validation of a magneto-optical detection device (Gazelle) for portable point-of-care Plasmodium vivax diagnosis

Hugo O. Valdivia, Priyaleela Thota, Greys Braga, Leonila Ricopa, Keare Barazorda, Carola Salas, Danett K. Bishop, Christie A. Joya

published 22 Jun 2021

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Molecular characterization of Cryptosporidium spp. from humans in Ethiopia

Ambachew W. Hailu, Abraham Degarege, Haileeyesus Adamu, Damien Costa, Venceslas Villier, Abdelmounaim Mouhajir, Loic Favennec, Romy Razakandrainibe, Beyene Petros

published 11 Jun 2021

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Validation of a novel FRET real-time PCR assay for simultaneous quantitative detection and discrimination of human Plasmodium parasites

Renate Schneider, Aline Lamien-Meda, Herbert Auer, Ursula Wiedermann-Schmidt, Peter L. Chiodini, Julia Walochnik

published 04 Jun 2021

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Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization

Clyde S. Manuel, Cassandra Suther, Matthew D. Moore, Lee-Ann Jaykus

published 08 Apr 2021

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Isolation, molecular characterization and phylogeny of Naegleria species in water bodies of North-Western Province, Sri Lanka

Nuwan Gunarathna, Anjalie Amarasinghe, Sunil Wijesundara, Devika Iddawela, Susiji Wickramasinghe

published 11 Mar 2021

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Vertical transmissibility of small ruminant lentivirus

Juscilânia Furtado Araújo, Alice Andrioli, Raymundo Rizaldo Pinheiro, Lucia Helena Sider, Ana Lídia Madeira de Sousa, Dalva Alana Aragão de Azevedo, Renato Mesquita Peixoto, Ana Milena Cesar Lima, Edgar Marques Damasceno, Samara Cristina Rocha Souza, Maria Fátima da Silva Teixeira

published 18 Nov 2020

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Combined PCR and MAT improves the early diagnosis of the biphasic illness leptospirosis

Noraini Philip, Norliza Bahtiar Affendy, Siti Norbaya Masri, Muhamad Yazli Yuhana, Leslie Thian Lung Than, Zamberi Sekawi, Vasantha Kumari Neela

published 11 Sep 2020

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A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

Eliška Vrbová, Lenka Mikalová, Linda Grillová, Petra Pospíšilová, Radim Strnadel, Eliška Dastychová, Martina Kojanová, Miluše Kreidlová, Daniela Vaňousová, Filip Rob, Přemysl Procházka, Alena Krchňáková, Vladimír Vašků, Vladana Woznicová, Monika Dvořáková Heroldová, Ivana Kuklová, Hana Zákoucká, David Šmajs

published 20 Aug 2020

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Point prevalence of asymptomatic Plasmodium infection and the comparison of microscopy, rapid diagnostic test and nested PCR for the diagnosis of asymptomatic malaria among children under 5 years in Ghana

Bismark Okyere, Alex Owusu-Ofori, Daniel Ansong, Rebecca Buxton, Scott Benson, Alex Osei-Akoto, Eddie-Williams Owiredu, Collins Adjei, Evans Xorse Amuzu, Joseph Marfo Boaheng, Ty Dickerson

published 27 Jul 2020

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