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Ligases

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet

GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis

Athanasios Lampropoulos, Zoran Sutikovic, [ ... ], Joachim Forner

Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase

Changlin Fu, William P. Donovan, [ ... ], Robert H. Cole

Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

John M. Pryor, Vladimir Potapov, [ ... ], Gregory J. S. Lohman

Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

Alexander Ulrich, Kasper R. Andersen, Thomas U. Schwartz

Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation

Sabrina Shore, Jordana M. Henderson, [ ... ], Richard I. Hogrefe

A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly

Theodore L. Roth, Ljiljana Milenkovic, Matthew P. Scott

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

Andreas I. Andreou, Naomi Nakayama

Analysis of the Role of Homology Arms in Gene-Targeting Vectors in Human Cells

Ayako Ishii, Aya Kurosawa, Shinta Saito, Noritaka Adachi

DNA Assembly in 3D Printed Fluidics

William G. Patrick, Alec A. K. Nielsen, [ ... ], David S. Kong

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost

Roger M. Benoit, Christian Ostermeier, [ ... ], Manfred Auer

TRAF6 Autoubiquitination-Independent Activation of the NFκB and MAPK Pathways in Response to IL-1 and RANKL

Matthew C. Walsh, Gregory K. Kim, [ ... ], Yongwon Choi

Ubiquitin Ligase RNF146 Regulates Tankyrase and Axin to Promote Wnt Signaling

Marinella G. Callow, Hoanh Tran, [ ... ], Mike Costa

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