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Genome engineering

Gene editing of PKLR gene in human hematopoietic progenitors through 5’ and 3’ UTR modified TALEN mRNA

Oscar Quintana-Bustamante, Sara Fañanas-Baquero, [ ... ], Jose C. Segovia

Integration of a FT expression cassette into CRISPR/Cas9 construct enables fast generation and easy identification of transgene-free mutants in Arabidopsis

Yuxin Cheng, Na Zhang, [ ... ], Shucai Wang

Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system

Yu-Wei Wu, Shih-Hung Yang, Myung Hwangbo, Kung-Hui Chu

CRISPR-Cas9 mediated CD133 knockout inhibits colon cancer invasion through reduced epithelial-mesenchymal transition

Wanlu Li, Mee-Yon Cho, [ ... ], Rackhyun Park

TET1 is an important transcriptional activator of TNFα expression in macrophages

Fangfang Sun, Irene Abreu-Rodriguez, [ ... ], Emmanuel Karouzakis

Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency

Ignacio García-Tuñón, Verónica Alonso-Pérez, [ ... ], Manuel Sánchez-Martín

Genetic characterisation of variants of the virulence plasmid, pSLT, in Salmonella enterica serovar Typhimurium provides evidence of a variety of evolutionary directions consistent with vertical rather than horizontal transmission

Lester Hiley, Rikki M. A. Graham, Amy V. Jennison

Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering

Oleg Raitskin, Christian Schudoma, Anthony West, Nicola J. Patron

CRISPR-cas gene-editing as plausible treatment of neuromuscular and nucleotide-repeat-expansion diseases: A systematic review

Haris Babačić, Aditi Mehta, Olivia Merkel, Benedikt Schoser

Generation of TGFBI knockout ABCG2+/ABCB5+ double-positive limbal epithelial stem cells by CRISPR/Cas9-mediated genome editing

Eung Kweon Kim, Seunghyuk Kim, Yong-Sun Maeng

Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts

Stijn Mertens, Brigida Gallone, [ ... ], Kevin J. Verstrepen

Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

Baptiste Castel, Laurence Tomlinson, [ ... ], Jonathan D. G. Jones

CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis

Marcus A. Price, Rita Cruz, [ ... ], Susan J. Rosser

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