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Genome engineering

indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events

Charles Hodgens, Zachary L. Nimchuk, Joseph J. Kieber

Systematic analysis of factors that improve homologous direct repair (HDR) efficiency in CRISPR/Cas9 technique

Mariateresa Di Stazio, Nicola Foschi, [ ... ], Adamo Pio d’Adamo

RNA-Seq Reveals Differential Gene Expression in Staphylococcus aureus with Single-Nucleotide Resolution

Joseph Osmundson, Scott Dewell, Seth A. Darst

Molecular Characterization of Salmonella enterica Serovar Aberdeen Negative for H₂S Production in China

Fuli Wu, Xuebin Xu, [ ... ], Shaofu Qiu

TALEN/CRISPR-Mediated eGFP Knock-In Add-On at the OCT4 Locus Does Not Impact Differentiation of Human Embryonic Stem Cells towards Endoderm

Nicole A. J. Krentz, Cuilan Nian, Francis C. Lynn

The CRISPR Associated Protein Cas4 Is a 5′ to 3′ DNA Exonuclease with an Iron-Sulfur Cluster

Jing Zhang, Taciana Kasciukovic, Malcolm F. White

p53 Gene Repair with Zinc Finger Nucleases Optimised by Yeast 1-Hybrid and Validated by Solexa Sequencing

Frank Herrmann, Mireia Garriga-Canut, [ ... ], Mark Isalan

Comparative Genomics of 12 Strains of Erwinia amylovora Identifies a Pan-Genome with a Large Conserved Core

Rachel A. Mann, Theo H. M. Smits, [ ... ], Brendan Rodoni

Creation of novel alleles of fragrance gene OsBADH2 in rice through CRISPR/Cas9 mediated gene editing

Shanthinie Ashokkumar, Deepa Jaganathan, [ ... ], Raveendran Muthurajan

Identification of Proteins Associated with an IFNγ-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR

Toshitsugu Fujita, Hodaka Fujii

Detection of genome-edited mutant clones by a simple competition-based PCR method

Takeshi Harayama, Howard Riezman

Magnetic Separation and Antibiotics Selection Enable Enrichment of Cells with ZFN/TALEN-Induced Mutations

Hyojin Kim, Myung-Sun Kim, [ ... ], Jin-Soo Kim

Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens

Mudra Hegde, Christine Strand, Ruth E. Hanna, John G. Doench

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