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Genome engineering

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Christopher T. Breunig, Tamara Durovic, [ ... ], Stefan H. Stricker

CRISPR Inhibition of Prophage Acquisition in Streptococcus pyogenes

Takashi Nozawa, Nayuta Furukawa, [ ... ], Ichiro Nakagawa

Sequencing of Seven Haloarchaeal Genomes Reveals Patterns of Genomic Flux

Erin A. Lynch, Morgan G. I. Langille, [ ... ], Marc T. Facciotti

Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases

Xavier Rios, Adrian W. Briggs, [ ... ], George M. Church

Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression

Fatwa Adikusuma, Chandran Pfitzner, Paul Quinton Thomas

Targeting G with TAL Effectors: A Comparison of Activities of TALENs Constructed with NN and NK Repeat Variable Di-Residues

Michelle L. Christian, Zachary L. Demorest, [ ... ], Daniel F. Voytas

Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish

Hirohito Kotani, Kiyohito Taimatsu, [ ... ], Atsuo Kawahara

Complete Genome Sequence of Thermus aquaticus Y51MC23

Phillip J. Brumm, Scott Monsma, [ ... ], David A. Mead

Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9

Lech Kaczmarczyk, Ylva Mende, Branko Zevnik, Walker S. Jackson

Haloquadratum walsbyi : Limited Diversity in a Global Pond

Mike L. Dyall-Smith, Friedhelm Pfeiffer, [ ... ], Dieter Oesterhelt

Evaluation of sgRNA Target Sites for CRISPR-Mediated Repression of TP53

Ingrid E. B. Lawhorn, Joshua P. Ferreira, Clifford L. Wang

Simple Genome Editing of Rodent Intact Embryos by Electroporation

Takehito Kaneko, Tomoji Mashimo

Efficient Methods for Targeted Mutagenesis in Zebrafish Using Zinc-Finger Nucleases: Data from Targeting of Nine Genes Using CompoZr or CoDA ZFNs

Raman Sood, Blake Carrington, [ ... ], Paul Liu

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