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Genome engineering

Effect of cryopreservation on A172 and U251 glioma cells infected with lentiviral vectors designed for CRISPR/Cas9-mediated aquaporin-8 knock-out

Hao Zhang, Shujuan Zhu, Yu Xing, Qian Liu, Zhen Guo, Ziling Cai, Zihao Shen, Qingqian Xia, Huajun Sheng

published 04 Mar 2022

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A meta-analysis of safety and efficacy of endovascular aneurysm repair in aneurysm patients with severe angulated infrarenal neck

Giulia Bernardini, Sarah Litterscheid, Giovanni Battista Torsello, Giovanni Federico Torsello, Efthymios Beropoulis, Denise Özdemir-van Brunschot

published 24 Feb 2022

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In silico analysis of potential off-target sites to gene editing for Mucopolysaccharidosis type I using the CRISPR/Cas9 system: Implications for population-specific treatments

Paola Carneiro, Martiela Vaz de Freitas, Ursula Matte

published 24 Jan 2022

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Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2

Jun Yang, Nilakshi Barua, Md. Nannur Rahman, Norman Lo, Tsz Fung Tsang, Xiao Yang, Paul K. S. Chan, Li Zhang, Margaret Ip

published 23 Dec 2021

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Multiplexed CRISPR-mediated engineering of protein secretory pathway genes in the thermotolerant methylotrophic yeast Ogataea thermomethanolica

Worarat Kruasuwan, Aekkachai Puseenam, Sutipa Tanapongpipat, Niran Roongsawang

published 23 Dec 2021

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Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration

Katherine S. Marsay, Sarah Greaves, Harsha Mahabaleshwar, Charmaine Min Ho, Henry Roehl, Peter N. Monk, Tom J. Carney, Lynda J. Partridge

published 30 Nov 2021

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Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

Marina A. Tyumentseva, Aleksandr I. Tyumentsev, Vasiliy G. Akimkin

published 09 Nov 2021

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A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station

Sarah Stahl-Rommel, David Li, Michelle Sung, Rebecca Li, Aarthi Vijayakumar, Kutay Deniz Atabay, G. Guy Bushkin, Christian L. Castro, Kevin D. Foley, D. Scott Copeland, Sarah L. Castro-Wallace, Ezequiel Alvarez Saavedra, Emily J. Gleason, Sebastian Kraves

published 30 Jun 2021

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Novel fluorescent-based reporter cell line engineered for monitoring homologous recombination events

Alejandra Bernardi, Dino Gobelli, Julia Serna, Paulina Nawrocka, Gabriel March-Rosselló, Antonio Orduña, Piotr Kozlowski, María Simarro, Miguel A. de la Fuente

published 30 Apr 2021

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Systematic analysis of factors that improve homologous direct repair (HDR) efficiency in CRISPR/Cas9 technique

Mariateresa Di Stazio, Nicola Foschi, Emmanouil Athanasakis, Paolo Gasparini, Adamo Pio d’Adamo

published 05 Mar 2021

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Genome-wide identification and analysis of highly specific CRISPR/Cas9 editing sites in pepper (Capsicum annuum L.)

Guanliang Li, Ziyan Zhou, Lingrui Liang, Zhao Song, Yafei Hu, Junjie Cui, Weili Chen, Kailin Hu, Jiaowen Cheng

published 29 Dec 2020

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P.F508del editing in cells from cystic fibrosis patients

Svetlana A. Smirnikhina, Ekaterina V. Kondrateva, Elmira P. Adilgereeva, Arina A. Anuchina, Milyausha I. Zaynitdinova, Yana S. Slesarenko, Angelina S. Ershova, Kirill D. Ustinov, Matvei I. Yasinovsky, Elena L. Amelina, Ekaterina S. Voronina, Valentina D. Yakushina, Vyacheslav Yu. Tabakov, Alexander V. Lavrov

published 11 Nov 2020

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Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

Hualan Liu, David S. Robinson, Zong-Yen Wu, Rita Kuo, Yasuo Yoshikuni, Ian K. Blaby, Jan-Fang Cheng

published 04 Nov 2020

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