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Genome engineering

Effect of cryopreservation on A172 and U251 glioma cells infected with lentiviral vectors designed for CRISPR/Cas9-mediated aquaporin-8 knock-out

Hao Zhang, Shujuan Zhu, [ ... ], Huajun Sheng

A meta-analysis of safety and efficacy of endovascular aneurysm repair in aneurysm patients with severe angulated infrarenal neck

Giulia Bernardini, Sarah Litterscheid, [ ... ], Denise Özdemir-van Brunschot

In silico analysis of potential off-target sites to gene editing for Mucopolysaccharidosis type I using the CRISPR/Cas9 system: Implications for population-specific treatments

Paola Carneiro, Martiela Vaz de Freitas, Ursula Matte

Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2

Jun Yang, Nilakshi Barua, [ ... ], Margaret Ip

Multiplexed CRISPR-mediated engineering of protein secretory pathway genes in the thermotolerant methylotrophic yeast Ogataea thermomethanolica

Worarat Kruasuwan, Aekkachai Puseenam, Sutipa Tanapongpipat, Niran Roongsawang

Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration

Katherine S. Marsay, Sarah Greaves, [ ... ], Lynda J. Partridge

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

Marina A. Tyumentseva, Aleksandr I. Tyumentsev, Vasiliy G. Akimkin

A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station

Sarah Stahl-Rommel, David Li, [ ... ], Sebastian Kraves

Novel fluorescent-based reporter cell line engineered for monitoring homologous recombination events

Alejandra Bernardi, Dino Gobelli, [ ... ], Miguel A. de la Fuente

Systematic analysis of factors that improve homologous direct repair (HDR) efficiency in CRISPR/Cas9 technique

Mariateresa Di Stazio, Nicola Foschi, [ ... ], Adamo Pio d’Adamo

Genome-wide identification and analysis of highly specific CRISPR/Cas9 editing sites in pepper (Capsicum annuum L.)

Guanliang Li, Ziyan Zhou, [ ... ], Jiaowen Cheng

P.F508del editing in cells from cystic fibrosis patients

Svetlana A. Smirnikhina, Ekaterina V. Kondrateva, [ ... ], Alexander V. Lavrov

Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

Hualan Liu, David S. Robinson, [ ... ], Jan-Fang Cheng

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