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Genome engineering

Effect of cryopreservation on A172 and U251 glioma cells infected with lentiviral vectors designed for CRISPR/Cas9-mediated aquaporin-8 knock-out

Hao Zhang, Shujuan Zhu, [ ... ], Huajun Sheng

A programmable DNA roadblock system using dCas9 and multivalent target sites

Emily K. Matozel, Stephen Parziale, Allen C. Price

Unveiling the endogenous CRISPR-Cas system in Pseudomonas aeruginosa PAO1

Javier Alejandro Delgado-Nungaray, Luis Joel Figueroa-Yáñez, [ ... ], Orfil Gonzalez-Reynoso

A PLA₂ deletion mutant using CRISPR/Cas9 coupled to RNASeq reveals insect immune genes associated with eicosanoid signaling

Mohammad Vatanparast, Mojtaba Esmaeily, David Stanley, Yonggyun Kim

CRISPR-Cas9 genome and long non-coding RNAs as a novel diagnostic index for prostate cancer therapy via liposomal-coated compounds

Mai O. Kadry, Rehab M. Abdel-Megeed

Evaluation of Peregrinus maidis transformer-2 as a target for CRISPR-based control

Yu-Hui Wang, Dina Espinoza Rivera, William Klobasa, Marcé D. Lorenzen

A statistical simulation model to guide the choices of analytical methods in arrayed CRISPR screen experiments

Chang Sik Kim, Jonathan Cairns, [ ... ], Xiang Zhang

Zygote cryobanking applied to CRISPR/Cas9 microinjection in mice

Geraldine Schlapp, María Noel Meikle, [ ... ], Martina Crispo

Hit screening with multivariate robust outlier detection

Hui Sun Leong, Tianhui Zhang, [ ... ], Steven Novick

Off-target sequence variations driven by the intrinsic properties of the Cas–sgRNA–DNA complex in genome editing

Celine Kurniawan, Takeshi Itoh

Proinsulin degradation and presentation of a proinsulin B-chain autoantigen involves ER-associated protein degradation (ERAD)-enzyme UBE2G2

Tom Cremer, Hanneke Hoelen, [ ... ], Emmanuel J. H. J. Wiertz

Evidence for gene essentiality in Leishmania using CRISPR

Wen-Wei Zhang, Greg Matlashewski

Different paths, same destination, yet diverse effects: Antecedent configurations and performance implications of digital transformation in pharmaceutical manufacturing enterprises——Evidence from Chinese listed companies

Bingxiao Shi, Chunlan Mou, Yiwen Zhang, Tiantian Cui

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