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Genome engineering

Predicting CRISPR-Cas12a guide efficiency for targeting using machine learning

Aidan O’Brien, Denis C. Bauer, Gaetan Burgio

Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Aya Bridgeland, Sudip Biswas, [ ... ], Endang M. Septiningsih

An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9

Mohammad J. Hossain, Tamara J. O’Connor

Generation of SNCA Cell Models Using Zinc Finger Nuclease (ZFN) Technology for Efficient High-Throughput Drug Screening

Warunee Dansithong, Sharan Paul, [ ... ], Duong P. Huynh

Functional Analysis of a Breast Cancer-Associated FGFR2 Single Nucleotide Polymorphism Using Zinc Finger Mediated Genome Editing

Luisa J. Robbez-Masson, Csaba Bödör, [ ... ], Richard P. Grose

Knockout of secondary alcohol dehydrogenase in Nocardia cholesterolicum NRRL 5767 by CRISPR/Cas9 genome editing technology

Jenq-Kuen Huang, Kadidia Samassekou, [ ... ], Lisa Wen

Granulin Knock Out Zebrafish Lack Frontotemporal Lobar Degeneration and Neuronal Ceroid Lipofuscinosis Pathology

Barbara Solchenberger, Claire Russell, [ ... ], Bettina Schmid

Cytotoxic Effects during Knock Out of Multiple Porcine Endogenous Retrovirus (PERV) Sequences in the Pig Genome by Zinc Finger Nucleases (ZFN)

Marwan Semaan, Daniel Ivanusic, Joachim Denner

Gene editing of PKLR gene in human hematopoietic progenitors through 5’ and 3’ UTR modified TALEN mRNA

Oscar Quintana-Bustamante, Sara Fañanas-Baquero, [ ... ], Jose C. Segovia

Genome-wide identification and analysis of highly specific CRISPR/Cas9 editing sites in pepper (Capsicum annuum L.)

Guanliang Li, Ziyan Zhou, [ ... ], Jiaowen Cheng

Diversity in a Polymicrobial Community Revealed by Analysis of Viromes, Endolysins and CRISPR Spacers

Michelle Davison, Todd J. Treangen, [ ... ], Devaki Bhaya

A Modified TALEN-Based Strategy for Rapidly and Efficiently Generating Knockout Mice for Kidney Development Studies

Yunhong Liu, Xiaoyan Lv, [ ... ], Qin Zhou

A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors

Koollawat Chupradit, Nontaphat Thongsin, Chatchai Tayapiwatana, Methichit Wattanapanitch

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