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Genome engineering

Detailed Phenotypic and Molecular Analyses of Genetically Modified Mice Generated by CRISPR-Cas9-Mediated Editing

Bijal A. Parikh, Diana L. Beckman, Swapneel J. Patel, J. Michael White, Wayne M. Yokoyama

published 14 Jan 2015

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Transcription Activator-Like Effector Nuclease (TALEN)-Mediated CLYBL Targeting Enables Enhanced Transgene Expression and One-Step Generation of Dual Reporter Human Induced Pluripotent Stem Cell (iPSC) and Neural Stem Cell (NSC) Lines

Trevor Cerbini, Ray Funahashi, Yongquan Luo, Chengyu Liu, Kyeyoon Park, Mahendra Rao, Nasir Malik, Jizhong Zou

published 14 Jan 2015

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High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

Holly R. Thomas, Stefanie M. Percival, Bradley K. Yoder, John M. Parant

published 11 Dec 2014

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CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome

Mohadeseh Mehrabian, Dylan Brethour, Sarah MacIsaac, Jin Kyu Kim, C . Geeth Gunawardana, Hansen Wang, Gerold Schmitt-Ulms

published 09 Dec 2014

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CRISPR Reveals a Distal Super-Enhancer Required for Sox2 Expression in Mouse Embryonic Stem Cells

Yan Li, Chloe M. Rivera, Haruhiko Ishii, Fulai Jin, Siddarth Selvaraj, Ah Young Lee, Jesse R. Dixon, Bing Ren

published 08 Dec 2014

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TALEN/CRISPR-Mediated eGFP Knock-In Add-On at the OCT4 Locus Does Not Impact Differentiation of Human Embryonic Stem Cells towards Endoderm

Nicole A. J. Krentz, Cuilan Nian, Francis C. Lynn

published 04 Dec 2014

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Genome Editing in Mouse Spermatogonial Stem/Progenitor Cells Using Engineered Nucleases

Danielle A. Fanslow, Stacey E. Wirt, Jenny C. Barker, Jon P. Connelly, Matthew H. Porteus, Christina Tenenhaus Dann

published 19 Nov 2014

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Evaluation of sgRNA Target Sites for CRISPR-Mediated Repression of TP53

Ingrid E. B. Lawhorn, Joshua P. Ferreira, Clifford L. Wang

published 14 Nov 2014

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Antibody-Free Magnetic Cell Sorting of Genetically Modified Primary Human CD4+ T Cells by One-Step Streptavidin Affinity Purification

Nicholas J. Matheson, Andrew A. Peden, Paul J. Lehner

published 31 Oct 2014

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MacSyFinder: A Program to Mine Genomes for Molecular Systems with an Application to CRISPR-Cas Systems

Sophie S. Abby, Bertrand Néron, Hervé Ménager, Marie Touchon, Eduardo P. C. Rocha

published 17 Oct 2014

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Genome Analysis of Environmental and Clinical P. aeruginosa Isolates from Sequence Type-1146

David Sánchez, Margarita Gomila, Antonio Bennasar, Jorge Lalucat, Elena García-Valdés

published 15 Oct 2014

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Dynamics of CRISPR Loci in Microevolutionary Process of Yersinia pestis Strains

Maria Paloma S. Barros, Camila T. França, Rosanny Holanda F. B. Lins, Milena Danda V. Santos, Ednaldo J. Silva, Maria Betânia M. Oliveira, Vladimir M. Silveira-Filho, Antônio M. Rezende, Valdir Q. Balbino, Tereza Cristina Leal-Balbino

published 29 Sep 2014

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Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation

Rolf B. Edvardsen, Sven Leininger, Lene Kleppe, Kai Ove Skaftnesmo, Anna Wargelius

published 25 Sep 2014

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