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Genome engineering

Different paths, same destination, yet diverse effects: Antecedent configurations and performance implications of digital transformation in pharmaceutical manufacturing enterprises——Evidence from Chinese listed companies

Bingxiao Shi, Chunlan Mou, Yiwen Zhang, Tiantian Cui

published 29 May 2025

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CRISPR genome editing using a combined positive and negative selection system

Ishrya Sharma, Kerisa Hall, Shannon Moonah

published 06 May 2025

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A simple validation and screening method for CRISPR/Cas9-mediated gene editing in mouse embryos to facilitate genetically modified mice production

Dawid Winiarczyk, Hossein Khodadadi, Paweł Leszczyński, Hiroaki Taniguchi

published 27 Mar 2025

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Unveiling the endogenous CRISPR-Cas system in Pseudomonas aeruginosa PAO1

Javier Alejandro Delgado-Nungaray, Luis Joel Figueroa-Yáñez, Eire Reynaga-Delgado, Ana Montserrat Corona-España, Orfil Gonzalez-Reynoso

published 31 Dec 2024

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Evidence for gene essentiality in Leishmania using CRISPR

Wen-Wei Zhang, Greg Matlashewski

published 30 Dec 2024

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GO-CRISPR: A highly controlled workflow to discover gene essentiality in loss-of-function screens

Pirunthan Perampalam, James I. McDonald, Frederick A. Dick

published 18 Dec 2024

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Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

published 14 Nov 2024

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CRISPR is easy: Exposure to Last Week Tonight enhances knowledge about gene editing

April A. Eichmeier, Michael A. Xenos

published 04 Oct 2024

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Hit screening with multivariate robust outlier detection

Hui Sun Leong, Tianhui Zhang, Adam Corrigan, Alessia Serrano, Ulrike Künzel, Niamh Mullooly, Ceri Wiggins, Yinhai Wang, Steven Novick

published 12 Sep 2024

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A statistical simulation model to guide the choices of analytical methods in arrayed CRISPR screen experiments

Chang Sik Kim, Jonathan Cairns, Valentina Quarantotti, Bogumil Kaczkowski, Yinhai Wang, Peter Konings, Xiang Zhang

published 20 Aug 2024

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A PLA₂ deletion mutant using CRISPR/Cas9 coupled to RNASeq reveals insect immune genes associated with eicosanoid signaling

Mohammad Vatanparast, Mojtaba Esmaeily, David Stanley, Yonggyun Kim

published 17 Jul 2024

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Zygote cryobanking applied to CRISPR/Cas9 microinjection in mice

Geraldine Schlapp, María Noel Meikle, Jorge Luis Pórfido, Alejo Menchaca, Martina Crispo

published 09 Jul 2024

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A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system

Rodrigo Moreno-Campos, Eileen W. Singleton, Rosa A. Uribe

published 29 May 2024

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