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Genome engineering

The Effect of Zinc and D-Penicillamine in a Stable Human Hepatoma ATP7B Knockout Cell Line

Gursimran Chandhok, Nadine Schmitt, [ ... ], Hartmut H. J. Schmidt

Novel Hypomorphic Alleles of the Mouse Tyrosinase Gene Induced by CRISPR-Cas9 Nucleases Cause Non-Albino Pigmentation Phenotypes

Anil K. Challa, Evan R. Boitet, [ ... ], Robert A. Kesterson

The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos

Kei Miyamoto, Ken-ichi T. Suzuki, [ ... ], J. B. Gurdon

TAL Effector Specificity for base 0 of the DNA Target Is Altered in a Complex, Effector- and Assay-Dependent Manner by Substitutions for the Tryptophan in Cryptic Repeat –1

Erin L. Doyle, Aaron W. Hummel, [ ... ], Adam J. Bogdanove

Crystal Structure of Streptococcus pyogenes Csn2 Reveals Calcium-Dependent Conformational Changes in Its Tertiary and Quaternary Structure

Yoon Koo, Du-kyo Jung, Euiyoung Bae

CRISPR is easy: Exposure to Last Week Tonight enhances knowledge about gene editing

April A. Eichmeier, Michael A. Xenos

Characterization of knockin mice at the Rosa26, Tac1 and Plekhg1 loci generated by homologous recombination in oocytes

Youmei Wu, María José Luna, [ ... ], James M. Pickel

CRISPR/Cas9-mediated correction of mutated copper transporter ATP7B

Michael Pöhler, Sarah Guttmann, [ ... ], Vanessa Sandfort

The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype

Sheran H. W. Law, Thomas D. Sargent

Genetic dissection of endothelial transcriptional activity of zebrafish aryl hydrocarbon receptors (AHRs)

Wade W. Sugden, Roberto C. Leonardo-Mendonça, Darío Acuña-Castroviejo, Arndt F. Siekmann

An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9

Mohammad J. Hossain, Tamara J. O’Connor

Establishment of pten knockout medaka with transcription activator–like effector nucleases (TALENs) as a model of PTEN deficiency disease

Yuriko Matsuzaki, Tetsushi Sakuma, Takashi Yamamoto, Hideyuki Saya

Predicting CRISPR-Cas12a guide efficiency for targeting using machine learning

Aidan O’Brien, Denis C. Bauer, Gaetan Burgio

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