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Genome engineering

Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish

Yunru Chen, Shiyang Zeng, Ruikun Hu, Xiangxiu Wang, Weilai Huang, Jiangfang Liu, Luying Wang, Guifen Liu, Ying Cao, Yong Zhang

published 11 Aug 2017

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IFNAR2-dependent gene expression profile induced by IFN-α in Pteropus alecto bat cells and impact of IFNAR2 knockout on virus infection

Qian Zhang, Lei-Ping Zeng, Peng Zhou, Aaron T. Irving, Shang Li, Zheng-Li Shi, Lin-Fa Wang

published 09 Aug 2017

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Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells

Shinsaku Tokuda, Toyohiro Hirai, Mikio Furuse

published 04 Aug 2017

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Zygotic vinculin is not essential for embryonic development in zebrafish

Mitchell K. L. Han, Gerard N. M. van der Krogt, Johan de Rooij

published 02 Aug 2017

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CRISPRpred: A flexible and efficient tool for sgRNAs on-target activity prediction in CRISPR/Cas9 systems

Md. Khaledur Rahman, M. Sohel Rahman

published 02 Aug 2017

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Reversible perturbations of gene regulation after genome editing in Drosophila cells

Stefan Kunzelmann, Klaus Förstemann

published 28 Jun 2017

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In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives

Hikmate Abriouel, Beatriz Pérez Montoro, María del Carmen Casado Muñoz, Charles W. Knapp, Antonio Gálvez, Nabil Benomar

published 26 Jun 2017

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Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells

Sanne Hindriksen, Arne J. Bramer, My Anh Truong, Martijn J. M. Vromans, Jasmin B. Post, Ingrid Verlaan-Klink, Hugo J. Snippert, Susanne M. A. Lens, Michael A. Hadders

published 22 Jun 2017

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No evidence for genome editing in mouse zygotes and HEK293T human cell line using the DNA-guided Natronobacterium gregoryi Argonaute (NgAgo)

Nay Chi Khin, Jenna L. Lowe, Lora M. Jensen, Gaetan Burgio

published 13 Jun 2017

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Detection of genome-edited mutant clones by a simple competition-based PCR method

Takeshi Harayama, Howard Riezman

published 06 Jun 2017

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Frameshift indels introduced by genome editing can lead to in-frame exon skipping

Simon Lalonde, Oliver A. Stone, Samuel Lessard, Adam Lavertu, Jessica Desjardins, Mélissa Beaudoin, Manuel Rivas, Didier Y. R. Stainier, Guillaume Lettre

published 01 Jun 2017

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Plasmid-free CRISPR/Cas9 genome editing in Plasmodium falciparum confirms mutations conferring resistance to the dihydroisoquinolone clinical candidate SJ733

Emily D. Crawford, Jenai Quan, Jeremy A. Horst, Daniel Ebert, Wesley Wu, Joseph L. DeRisi

published 22 May 2017

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CRISPR/Cas9-mediated targeted mutagenesis in grape

Ikuko Nakajima, Yusuke Ban, Akifumi Azuma, Noriyuki Onoue, Takaya Moriguchi, Toshiya Yamamoto, Seiichi Toki, Masaki Endo

published 18 May 2017

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