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Genome engineering

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

Megan Basila, Melissa L. Kelley, Anja van Brabant Smith

published 27 Nov 2017

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indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events

Charles Hodgens, Zachary L. Nimchuk, Joseph J. Kieber

published 15 Nov 2017

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Establishment of pten knockout medaka with transcription activator–like effector nucleases (TALENs) as a model of PTEN deficiency disease

Yuriko Matsuzaki, Tetsushi Sakuma, Takashi Yamamoto, Hideyuki Saya

published 20 Oct 2017

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CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna

Hitoshi Kumagai, Takashi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

published 18 Oct 2017

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Diversity and microevolution of CRISPR loci in Helicobacter cinaedi

Junko Tomida, Yuji Morita, Keigo Shibayama, Ken Kikuchi, Tomohiro Sawa, Takaaki Akaike, Yoshiaki Kawamura

published 13 Oct 2017

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Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9

Wenjun Zhou, Yongjie Wan, Rihong Guo, Mingtian Deng, Kaiping Deng, Zhen Wang, Yanli Zhang, Feng Wang

published 10 Oct 2017

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Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata

Thomas W. Kirchner, Markus Niehaus, Thomas Debener, Manfred K. Schenk, Marco Herde

published 22 Sep 2017

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Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia

Christopher D. Johnston, Chelsey A. Skeete, Alexey Fomenkov, Richard J. Roberts, Susan R. Rittling

published 21 Sep 2017

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Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome

Eric Weh, Hideyuki Takeuchi, Sanaa Muheisen, Robert S. Haltiwanger, Elena V. Semina

published 19 Sep 2017

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CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments

Qingyi Cao, Jian Ma, Chen-Hao Chen, Han Xu, Zhi Chen, Wei Li, X. Shirley Liu

published 05 Sep 2017

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Genetic dissection of endothelial transcriptional activity of zebrafish aryl hydrocarbon receptors (AHRs)

Wade W. Sugden, Roberto C. Leonardo-Mendonça, Darío Acuña-Castroviejo, Arndt F. Siekmann

published 17 Aug 2017

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Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells

Olaf Voets, Frans Tielen, Edo Elstak, Julian Benschop, Max Grimbergen, Jan Stallen, Richard Janssen, Andre van Marle, Christian Essrich

published 11 Aug 2017

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Comparative pathogenomics of Clostridium tetani

Jonathan E. Cohen, Rong Wang, Rong-Fong Shen, Wells W. Wu, James E. Keller

published 11 Aug 2017

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