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Genome engineering

CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna

Hitoshi Kumagai, Takashi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

published 18 Oct 2017

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Diversity and microevolution of CRISPR loci in Helicobacter cinaedi

Junko Tomida, Yuji Morita, Keigo Shibayama, Ken Kikuchi, Tomohiro Sawa, Takaaki Akaike, Yoshiaki Kawamura

published 13 Oct 2017

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Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9

Wenjun Zhou, Yongjie Wan, Rihong Guo, Mingtian Deng, Kaiping Deng, Zhen Wang, Yanli Zhang, Feng Wang

published 10 Oct 2017

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Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata

Thomas W. Kirchner, Markus Niehaus, Thomas Debener, Manfred K. Schenk, Marco Herde

published 22 Sep 2017

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Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia

Christopher D. Johnston, Chelsey A. Skeete, Alexey Fomenkov, Richard J. Roberts, Susan R. Rittling

published 21 Sep 2017

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Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome

Eric Weh, Hideyuki Takeuchi, Sanaa Muheisen, Robert S. Haltiwanger, Elena V. Semina

published 19 Sep 2017

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CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments

Qingyi Cao, Jian Ma, Chen-Hao Chen, Han Xu, Zhi Chen, Wei Li, X. Shirley Liu

published 05 Sep 2017

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Genetic dissection of endothelial transcriptional activity of zebrafish aryl hydrocarbon receptors (AHRs)

Wade W. Sugden, Roberto C. Leonardo-Mendonça, Darío Acuña-Castroviejo, Arndt F. Siekmann

published 17 Aug 2017

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Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells

Olaf Voets, Frans Tielen, Edo Elstak, Julian Benschop, Max Grimbergen, Jan Stallen, Richard Janssen, Andre van Marle, Christian Essrich

published 11 Aug 2017

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Comparative pathogenomics of Clostridium tetani

Jonathan E. Cohen, Rong Wang, Rong-Fong Shen, Wells W. Wu, James E. Keller

published 11 Aug 2017

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Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish

Yunru Chen, Shiyang Zeng, Ruikun Hu, Xiangxiu Wang, Weilai Huang, Jiangfang Liu, Luying Wang, Guifen Liu, Ying Cao, Yong Zhang

published 11 Aug 2017

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IFNAR2-dependent gene expression profile induced by IFN-α in Pteropus alecto bat cells and impact of IFNAR2 knockout on virus infection

Qian Zhang, Lei-Ping Zeng, Peng Zhou, Aaron T. Irving, Shang Li, Zheng-Li Shi, Lin-Fa Wang

published 09 Aug 2017

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Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells

Shinsaku Tokuda, Toyohiro Hirai, Mikio Furuse

published 04 Aug 2017

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