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Gene expression and vector techniques

pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2

Inês M. Luís, Mariana Parada, João B. Vicente, Isabel A. Abreu

published 07 Jul 2025

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Reciprocal effects of conditioned medium on gene and protein expression of limbal epithelial cells and limbal fibroblasts in congenital aniridia

Maximilian Berger, Nóra Szentmáry, Tim Berger, Julia Zimmermann, Simon Trusen, Berthold Seitz, Fabian N. Fries, Shweta Suiwal, Maryam Amini, Tanja Stachon

published 07 Jul 2025

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Proteome remodeling in the zoospore-to-vegetative cell transition of the stramenopile Aurantiochytrium limacinum reveals candidate ectoplasmic network proteins

Alejandro Gil-Gomez, Ben Leyland, Anbarasu Karthikaichamy, Rebecca C. Adikes, David Q. Matus, Joshua S. Rest, Jackie L. Collier

published 02 Jul 2025

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PLZF promotes compensatory lung growth by increasing HPMEC proliferation and angiogenesis

Jing Peng, Liang Ma, Zhonghui Wang, Yaxi Du, Qunfen Tao, Qiongchuan Wang, Li Zhao

published 02 Jul 2025

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Copper supplementation enhances pigmentation and induces dopamine production in ARPE19

Hironori Uehara, Baila Shakaib, Sangeetha Ravi Kumar, Bonnie Archer, Balamurali Ambati

published 01 Jul 2025

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Analysis of disease resistance of ZmERS4 -overexpressing rice

Wanyuan Huang, Jun Ning, Bo Su, Wenjie Liu, Ting Ding

published 01 Jul 2025

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Proteomic and phosphoproteomic analysis of rabies pathogenesis in the clinical canine brain and identification of a kinase inhibitor as a potential repurposed antiviral agent

Peerut Chienwichai, Kunjimas Ketsuwan, Boonlert Lumlertdacha, Chanon Fa-Ngoen, Punchaya Supasawat, Rojjanaporn Pulmanausahakul, Promsin Masrinoul, Tipparat Thiangtrongjit, Onrapak Reamtong

published 27 Jun 2025

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Ivermectin inhibits epithelial-to-mesenchymal transition via Wnt signaling in endocrine-resistant breast cancer cells

Kitiya Rujimongkon, Patthamapon Adchariyasakulchai, Phum Meeprasertskul, Wannarasmi Ketchart

published 26 Jun 2025

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Investigating the cardiorespiratory fitness gene COX7A2L in cardiomyocytes: Viability and mitochondrial function

Ada Nilsen Nordeidet, Gurdeep S. A. Marwarha, Øystein Røsand, Victoria Johansen, Karin Garten, Morten A. Høydal, Mette Langaas, Anja Bye

published 25 Jun 2025

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Dynamic CYP2E1 expression and metabolic activity changes in male rats during immune liver injury and sex differences in alcohol metabolism

Jiayi Zhang, Yingying Cao, Ziqi Jin, Runa A, Xiaoxue Wang, Lingyu Zhang, Yingqi Hu, Yongzhi Xue

published 18 Jun 2025

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Effect of retinoic acid treatment on the retinoic acid signaling pathway in a human siRNA-based aniridia limbal epithelial cell model, in vitro

Shao-Lun Hsu, Tanja Stachon, Fabian N. Fries, Zhen Li, Shuailin Li, Shanhe Liu, Berthold Seitz, Swarnali Kundu, Maryam Amini, Shweta Suiwal, Nóra Szentmáry

published 18 Jun 2025

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Modulating autophagy in KRAS mutant colorectal cancer using combination of oncolytic reovirus and carbamazepine

Aaron Shaykevich, Danbee Chae, Isaac Silverman, Sanjay Goel, Radhashree Maitra

published 17 Jun 2025

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Titration-WB: A methodology for accurate quantitative protein determination overcoming reproducibility errors

Alice Maestri, Ewa Ehrenborg, Olivera Werngren, Maria Olin, Carolina E. Hagberg, Matteo Pedrelli, Paolo Parini

published 12 Jun 2025

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