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Gene amplification

Feasibility of developing reliable gene expression modules from FFPE derived RNA profiled on Affymetrix arrays

Vinu Jose, Debora Fumagalli, Françoise Rothé, Samira Majjaj, Sherene Loi, Stefan Michiels, Christos Sotiriou

published 31 Aug 2018

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Sequencing of Treponema pallidum subsp. pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material

Linda Grillová, Lorenzo Giacani, Lenka Mikalová, Michal Strouhal, Radim Strnadel, Christina Marra, Arturo Centurion-Lara, Lucy Poveda, Giancarlo Russo, Darina Čejková, Vladimír Vašků, Jan Oppelt, David Šmajs

published 21 Aug 2018

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Development of a novel real-time polymerase chain reaction assay for the quantitative detection of Nipah virus replicative viral RNA

Kenneth S. Jensen, Ricky Adams, Richard S. Bennett, John Bernbaum, Peter B. Jahrling, Michael R. Holbrook

published 19 Jun 2018

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Identification of reference genes for RT-qPCR in the Antarctic moss Sanionia uncinata under abiotic stress conditions

Mira Park, Soon Gyu Hong, Hyun Park, Byeong-ha Lee, Hyoungseok Lee

published 19 Jun 2018

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Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples

Stine H. Kresse, Heidi M. Namløs, Susanne Lorenz, Jeanne-Marie Berner, Ola Myklebost, Bodil Bjerkehagen, Leonardo A. Meza-Zepeda

published 17 May 2018

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Massively parallel sequencing of micro-manipulated cells targeting a comprehensive panel of disease-causing genes: A comparative evaluation of upstream whole-genome amplification methods

Lieselot Deleye, Yannick Gansemans, Dieter De Coninck, Filip Van Nieuwerburgh, Dieter Deforce

published 26 Apr 2018

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A streamlined workflow for single-cells genome-wide copy-number profiling by low-pass sequencing of LM-PCR whole-genome amplification products

Alberto Ferrarini, Claudio Forcato, Genny Buson, Paola Tononi, Valentina del Monaco, Mario Terracciano, Chiara Bolognesi, Francesca Fontana, Gianni Medoro, Rui Neves, Birte Möhlendick, Karim Rihawi, Andrea Ardizzoni, Semini Sumanasuriya, Penny Flohr, Maryou Lambros, Johann de Bono, Nikolas H. Stoecklein, Nicolò Manaresi

published 01 Mar 2018

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Single cell on-chip whole genome amplification via micropillar arrays for reduced amplification bias

Harvey C. Tian, Jaime J. Benitez, Harold G. Craighead

published 12 Feb 2018

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Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva

Maite Sabalza, Rubina Yasmin, Cheryl A. Barber, Talita Castro, Daniel Malamud, Beum Jun Kim, Hui Zhu, Richard A. Montagna, William R. Abrams

published 05 Feb 2018

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Prognostic significance of cyclin D1 protein expression and gene amplification in invasive breast carcinoma

Angela B. Ortiz, Diego Garcia, Yolanda Vicente, Magda Palka, Carmen Bellas, Paloma Martin

published 15 Nov 2017

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Single primer isothermal amplification (SPIA) combined with next generation sequencing provides complete bovine coronavirus genome coverage and higher sequence depth compared to sequence-independent single primer amplification (SISPA)

Mette Myrmel, Veslemøy Oma, Mamata Khatri, Hanne H. Hansen, Maria Stokstad, Mikael Berg, Anne-Lie Blomström

published 07 Nov 2017

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High SLC4A11 expression is an independent predictor for poor overall survival in grade 3/4 serous ovarian cancer

Lianzhi Qin, Ting Li, Yuhua Liu

published 01 Nov 2017

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Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Amanda E. Calvert, Brad J. Biggerstaff, Nathan A. Tanner, Molly Lauterbach, Robert S. Lanciotti

published 25 Sep 2017

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