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Extraction techniques

Optimizing direct RT-LAMP to detect transmissible SARS-CoV-2 from primary nasopharyngeal swab samples

Dawn M. Dudley, Christina M. Newman, Andrea M. Weiler, Mitchell D. Ramuta, Cecilia G. Shortreed, Anna S. Heffron, Molly A. Accola, William M. Rehrauer, Thomas C. Friedrich, David H. O’Connor

published 31 Dec 2020

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A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2

Tomislav Maricic, Olaf Nickel, Ayinuer Aximu-Petri, Elena Essel, Marie Gansauge, Philipp Kanis, Dominik Macak, Julia Richter, Stephan Riesenberg, Lukas Bokelmann, Hugo Zeberg, Matthias Meyer, Stephan Borte, Svante Pääbo

published 31 Dec 2020

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Optimization of ultrasonic-assisted extraction of polysaccharides and triterpenoids from the medicinal mushroom Ganoderma lucidum and evaluation of their in vitro antioxidant capacities

Shizhong Zheng, Weirui Zhang, Shengrong Liu

published 31 Dec 2020

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Detection of meat from horse, donkey and their hybrids (mule/hinny) by duplex real-time fluorescent PCR

Dan Wang, Liping Wang, Chenyu Xue, Yuebei Han, Hejing Li, Jianqiang Geng, Jiang Jie

published 29 Dec 2020

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Consistent patterns in 16S and 18S microbial diversity from the shells of the common and widespread red-eared slider turtle (Trachemys scripta)

Matthew Parks, Cameron Kedy, Casey Skalla

published 28 Dec 2020

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Influence of sediment and stream transport on detecting a source of environmental DNA

Meredith B. Nevers, Kasia Przybyla-Kelly, Dawn Shively, Charles C. Morris, Joshua Dickey, Murulee N. Byappanahalli

published 28 Dec 2020

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A simplified SARS-CoV-2 detection protocol for research laboratories

Sean Paz, Christopher Mauer, Anastasia Ritchie, Janet D. Robishaw, Massimo Caputi

published 18 Dec 2020

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A strategic expression method of miR-29b and its anti-fibrotic effect based on RNA-sequencing analysis

Xiaoming Fan, Yingnyu Gao, Xiaolu Zhang, Haroon Y. Lughmani, David J. Kennedy, Steven T. Haller, Sandrine V. Pierre, Joseph I. Shapiro, Jiang Tian

published 17 Dec 2020

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Demonstration of a fast and easy sample-to-answer protocol for tuberculosis screening in point-of-care settings: A proof of concept study

Nasir Ali, Graziele Lima Bello, Maria Lúcia Rosa Rossetti, Marco Aurelio Krieger, Alexandre Dias Tavares Costa

published 14 Dec 2020

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Selected reaction monitoring for the quantification of Escherichia coli ribosomal proteins

Yuishin Kosaka, Wataru Aoki, Megumi Mori, Shunsuke Aburaya, Yuta Ohtani, Hiroyoshi Minakuchi, Mitsuyoshi Ueda

published 14 Dec 2020

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Quantitative PCR assays to detect whales, rockfish, and common murre environmental DNA in marine water samples of the Northeastern Pacific

Elizabeth A. Andruszkiewicz, Kevan M. Yamahara, Collin J. Closek, Alexandria B. Boehm

published 02 Dec 2020

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Quality assessment of RNA in long-term storage: The All Our Families biorepository

Nikki L. Stephenson, Kylie K. Hornaday, Chelsea T. A. Doktorchik, Andrew W. Lyon, Suzanne C. Tough, Donna M. Slater

published 01 Dec 2020

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Alternative AKT2 splicing produces protein lacking the hydrophobic motif regulatory region

Guido Plotz, Laura A. Lopez-Garcia, Angela Brieger, Stefan Zeuzem, Ricardo M. Biondi

published 30 Nov 2020

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