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Extraction techniques

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

Thomas G. W. Graham, Claire Dugast-Darzacq, Gina M. Dailey, Xammy Huu Wrynla, Erik Van Dis, Meagan N. Esbin, Abrar Abidi, Sarah A. Stanley, Xavier Darzacq, Robert Tjian

published 03 Feb 2021

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Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp

Paula Finamore-Araujo, Amanda Faier-Pereira, Carlos Ramon do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy de Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio Cruz Moreira

published 02 Feb 2021

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Testing the effect of pollen exine rupture on metabarcoding with Illumina sequencing

Stephanie J. Swenson, Birgit Gemeinholzer

published 02 Feb 2021

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A comparison of the effectiveness of QuEChERS, FaPEx and a modified QuEChERS method on the determination of organochlorine pesticides in ginseng

Pennante Bruce-Vanderpuije, David Megson, Song-Hee Ryu, Geun-Hyoung Choi, Sang-Won Park, Byung-Seok Kim, Jin Hyo Kim, Hyo-Sub Lee

published 29 Jan 2021

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Effects of a tart cherry (Prunus cerasus L.) phenolic extract on Porphyromonas gingivalis and its ability to impair the oral epithelial barrier

Amel Ben Lagha, Geneviève Pellerin, Katy Vaillancourt, Daniel Grenier

published 26 Jan 2021

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Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry

Prasanna Channathodiyil, Jonathan Houseley

published 22 Jan 2021

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Policymaking through a knowledge lens: Using the embodied-enacted-inscribed knowledge framework to illuminate the transfer of knowledge in a mental health policy consultation process – A South African case study

Debra Leigh Marais, Inge Petersen, Michael Quayle

published 13 Jan 2021

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Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification

Pierre Garneret, Etienne Coz, Elian Martin, Jean-Claude Manuguerra, Elodie Brient-Litzler, Vincent Enouf, Daniel Felipe González Obando, Jean-Christophe Olivo-Marin, Fabrice Monti, Sylvie van der Werf, Jessica Vanhomwegen, Patrick Tabeling

published 11 Jan 2021

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Detection and differentiation of Burkholderia species with pathogenic potential in environmental soil samples

Sujintana Janesomboon, Veerachat Muangsombut, Varintip Srinon, Chatruthai Meethai, Chayada S. Tharinjaroen, Premjit Amornchai, Patoo Withatanung, Narisara Chantratita, Mark Mayo, Vanaporn Wuthiekanun, Bart J. Currie, Joanne M. Stevens, Sunee Korbsrisate

published 07 Jan 2021

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Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor

Thanawat Sridapan, Wanida Tangkawsakul, Tavan Janvilisri, Wansika Kiatpathomchai, Sirintip Dangtip, Natharin Ngamwongsatit, Duangjai Nacapricha, Puey Ounjai, Surang Chankhamhaengdecha

published 07 Jan 2021

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Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Yee Ling Lau, Ilyiana binti Ismail, Nur Izati binti Mustapa, Meng Yee Lai, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Maria Kahar Bador Abdul Kahar, Jennifer Chong, Pik Pin Goh

published 06 Jan 2021

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Personizing the prediction of future susceptibility to a specific disease

Kamal Taha, Ramana Davuluri, Paul Yoo, Jesse Spencer

published 06 Jan 2021

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Assessment of two DNA extraction kits for profiling poultry respiratory microbiota from multiple sample types

Michael E. C. Abundo, John M. Ngunjiri, Kara J. M. Taylor, Hana Ji, Amir Ghorbani, Mahesh K. C., Bonnie P. Weber, Timothy J. Johnson, Chang-Won Lee

published 06 Jan 2021

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