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Extraction techniques

Evaluation of sample pooling for screening of SARS CoV-2

Andargachew Mulu, Dawit Hailu Alemayehu, Fekadu Alemu, Dessalegn Abeje Tefera, Sinknesh Wolde, Gebeyehu Aseffa, Tamrayehu Seyoum, Meseret Habtamu, Alemseged Abdissa, Abebe Genetu Bayih, Getachew Tesfaye Beyene

published 26 Feb 2021

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From e-voucher to genomic data: Preserving archive specimens as demonstrated with medically important mosquitoes (Diptera: Culicidae) and kissing bugs (Hemiptera: Reduviidae)

Silvia Andrade Justi, John Soghigian, David B. Pecor, Laura Caicedo-Quiroga, Wiriya Rutvisuttinunt, Tao Li, Lori Stevens, Patricia L. Dorn, Brian Wiegmann, Yvonne-Marie Linton

published 25 Feb 2021

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PFP-WGAN: Protein function prediction by discovering Gene Ontology term correlations with generative adversarial networks

Seyyede Fatemeh Seyyedsalehi, Mahdieh Soleymani, Hamid R. Rabiee, Mohammad R. K. Mofrad

published 25 Feb 2021

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Phenol-chloroform-based RNA purification for detection of SARS-CoV-2 by RT-qPCR: Comparison with automated systems

Henrik Dimke, Sanne L. Larsen, Marianne N. Skov, Hanne Larsen, Gitte N. Hartmeyer, Jesper B. Moeller

published 24 Feb 2021

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Saliva as a testing specimen with or without pooling for SARS-CoV-2 detection by multiplex RT-PCR test

Qing Sun, Jonathan Li, Hui Ren, Larry Pastor, Yulia Loginova, Roberta Madej, Kristopher Taylor, Joseph K. Wong, Zhao Zhang, Aiguo Zhang, Chuanyi M. Lu, Michael Y. Sha

published 23 Feb 2021

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Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method

Takuyu Hashiguchi, Masatsugu Hashiguchi, Hidenori Tanaka, Koki Fukushima, Takahiro Gondo, Ryo Akashi

published 18 Feb 2021

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Impact of pre-analytic step duration on molecular diagnosis of toxoplasmosis for five types of biological samples

Marie-Pierre Brenier-Pinchart, Emmanuelle Varlet-Marie, Florence Robert-Gangneux, Denis Filisetti, Juliette Guitard, Yvon Sterkers, Hélène Yera, Hervé Pelloux, Patrick Bastien

published 17 Feb 2021

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A DNA metabarcoding approach for recovering plankton communities from archived samples fixed in formalin

Takuhei Shiozaki, Fumihiro Itoh, Yuu Hirose, Jonaotaro Onodera, Akira Kuwata, Naomi Harada

published 17 Feb 2021

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Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection

Fernando Lázaro-Perona, Carlos Rodriguez-Antolín, Marina Alguacil-Guillén, Almudena Gutiérrez-Arroyo, Jesús Mingorance, Julio García-Rodriguez, on behalf of the SARS-CoV-2 Working Group

published 16 Feb 2021

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Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

C. H. W. M. R. Bhagya Chandrasekara, D. Nathasha U. Naranpanawa, B. Supun Bandusekara, D. K. N. G. Pushpakumara, D. Siril A. Wijesundera, Pradeepa C. G. Bandaranayake

published 10 Feb 2021

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Comparative performance of SARS-CoV-2 real-time PCR diagnostic assays on samples from Lagos, Nigeria

Chika Kingsley Onwuamah, Azuka Patrick Okwuraiwe, Olumuyiwa B. Salu, Joseph O. Shaibu, Nnaemeka Ndodo, Samuel O. Amoo, Leona C. Okoli, Fehintola A. Ige, Rahaman A. Ahmed, Munir Akinwale Bankole, Judith O. Sokei, Bamidele Paul Mutiu, James Ayorinde, Babatunde Akeem Saka, Celestina Obiekea, Nwando Mba, Richard A. Adegbola, Sunday Omilabu, Chikwe Ihekweazu, Babatunde Lawal Salako, Rosemary Audu

published 04 Feb 2021

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Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

Thomas G. W. Graham, Claire Dugast-Darzacq, Gina M. Dailey, Xammy Huu Wrynla, Erik Van Dis, Meagan N. Esbin, Abrar Abidi, Sarah A. Stanley, Xavier Darzacq, Robert Tjian

published 03 Feb 2021

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Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp

Paula Finamore-Araujo, Amanda Faier-Pereira, Carlos Ramon do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy de Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio Cruz Moreira

published 02 Feb 2021

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