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Extraction techniques

Detection of six soil-transmitted helminths in human stool by qPCR- a systematic workflow

Kristy I. Azzopardi, Myra Hardy, Ciara Baker, Rhian Bonnici, Stacey Llewellyn, James S. McCarthy, Rebecca J. Traub, Andrew C. Steer

published 30 Sep 2021

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Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk

Sanoji Wijenayake, Shafinaz Eisha, Zoya Tawhidi, Michael A. Pitino, Michael A. Steele, Alison S. Fleming, Patrick O. McGowan

published 30 Sep 2021

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A workflow for the relative quantification of multiple fish species from oceanic water samples using environmental DNA (eDNA) to support large-scale fishery surveys

Ana Ramón-Laca, Abigail Wells, Linda Park

published 27 Sep 2021

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A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections

Meng-Jia Lau, Ary A. Hoffmann, Nancy M. Endersby-Harshman

published 23 Sep 2021

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Protocol for safe, affordable, and reproducible isolation and quantitation of SARS-CoV-2 RNA from wastewater

Monica Trujillo, Kristen Cheung, Anna Gao, Irene Hoxie, Sherin Kannoly, Nanami Kubota, Kaung Myat San, Davida S. Smyth, John J. Dennehy

published 23 Sep 2021

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WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva

David Ng, Ana Pinharanda, Merly C. Vogt, Ashok Litwin-Kumar, Kyle Stearns, Urvashi Thopte, Enrico Cannavo, Armen Enikolopov, Felix Fiederling, Stylianos Kosmidis, Barbara Noro, Ines Rodrigues-Vaz, Hani Shayya, Peter Andolfatto, Darcy S. Peterka, Tanya Tabachnik, Jeanine D’Armiento, Monica Goldklang, Andres Bendesky

published 16 Sep 2021

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Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue

Alireza Saraji, Anne Offermann, Janine Stegmann-Frehse, Katharina Hempel, Duan Kang, Rosemarie Krupar, Christian Watermann, Danny Jonigk, Mark Philipp Kühnel, Jutta Kirfel, Sven Perner, Verena Sailer

published 16 Sep 2021

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Intraspecific genetic variability and diurnal activity affect environmental DNA detection in Japanese eel

Sayaka Takahashi, Shingo Takada, Hiroki Yamanaka, Reiji Masuda, Akihide Kasai

published 16 Sep 2021

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Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Maria Jose Lista, Pedro M. Matos, Thomas J. A. Maguire, Kate Poulton, Elena Ortiz-Zapater, Robert Page, Helin Sertkaya, Ana M. Ortega-Prieto, Edward Scourfield, Aoife M. O’Byrne, Clement Bouton, Ruth E. Dickenson, Mattia Ficarelli, Jose M. Jimenez-Guardeño, Mark Howard, Gilberto Betancor, Rui Pedro Galao, Suzanne Pickering, Adrian W. Signell, Harry Wilson, Penelope Cliff, Mark Tan Kia Ik, Amita Patel, Eithne MacMahon, Emma Cunningham, Katie Doores, Monica Agromayor, Juan Martin-Serrano, Esperanza Perucha, Hannah E. Mischo, Manu Shankar-Hari, Rahul Batra, Jonathan Edgeworth, Mark Zuckerman, Michael H. Malim, Stuart Neil, Rocio Teresa Martinez-Nunez

published 15 Sep 2021

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Deciphering the low abundance microbiota of presumed aseptic hip and knee implants

Charles Carr, Hannah Wilcox, Jeremy P. Burton, Sharanya Menon, Kait F. Al, David O’Gorman, Brent A. Lanting, Edward M. Vasarhelyi, Michael Neufeld, Matthew G. Teeter

published 14 Sep 2021

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Olive fruit fly and its obligate symbiont Candidatus Erwinia dacicola: Two new symbiont haplotypes in the Mediterranean basin

Tânia Nobre

published 08 Sep 2021

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Self-collection and pooling of samples as resources-saving strategies for RT-PCR-based SARS-CoV-2 surveillance, the example of travelers in French Polynesia

Maite Aubry, Iotefa Teiti, Anita Teissier, Vaea Richard, Teheipuaura Mariteragi-Helle, Kiyojiken Chung, Farah Deen, Tuterarii Paoaafaite, Van-Mai Cao-Lormeau

published 02 Sep 2021

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Evaluation of DNA extraction protocols from liquid-based cytology specimens for studying cervical microbiota

Takeo Shibata, Mayumi Nakagawa, Hannah N. Coleman, Sarah M. Owens, William W. Greenfield, Toshiyuki Sasagawa, Michael S. Robeson II

published 30 Aug 2021

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