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Extraction techniques

The role of Atg16 in autophagy, anthocyanin biosynthesis, and programmed cell death in leaves of the lace plant (Aponogeton madagascariensis)

Nathan M. Rowarth, Adrian N. Dauphinee, Christian R. Lacroix, Arunika H. L. A. N. Gunawardena

published 16 Feb 2023

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Development of a cost-effective, morphology-preserving method for DNA isolation from bulk invertebrate trap catches: Tephritid fruit flies as an exemplar

Elizabeth V. Fowler, Melissa L. Starkie, Xiaocheng Zhu, Alexander M. Piper, Arati Agarwal, Lea Rako, Alexandra Gardiner, Sybilla Oczkowicz, David Gopurenko, Mark K. Schutze, Mark J. Blacket

published 15 Feb 2023

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Validation of saline, PBS and a locally produced VTM at varying storage conditions to detect the SARS-CoV-2 virus by qRT-PCR

Caroline Ngetsa, Victor Osoti, Dorcas Okanda, Faith Marura, Krupali Shah, Henry Karanja, Daisy Mugo, John Gitonga, Martin Mutunga, Clement Lewa, Benedict Orindi, Philip Bejon, Lynette Isabella Ochola-Oyier

published 13 Feb 2023

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Gallbladder microbiota in healthy dogs and dogs with mucocele formation

Jody L. Gookin, Ashley N. Hartley, Kathleen M. Aicher, Kyle G. Mathews, Rachel Cullen, John M. Cullen, Benjamin J. Callahan, Devorah M. Stowe, Gabriela S. Seiler, Megan E. Jacob, Jason W. Arnold, M. Andrea Azcarate-Peril, Stephen H. Stauffer

published 10 Feb 2023

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Greater than pH 8: The pH dependence of EDTA as a preservative of high molecular weight DNA in biological samples

Mia L. DeSanctis, Elizabeth A. Soranno, Ella Messner, Ziyu Wang, Elena M. Turner, Rosalia Falco, Hannah J. Appiah-Madson, Daniel L. Distel

published 23 Jan 2023

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The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein

Abdallah Mound, Gautier Goormachtigh, Fabrice Bray, Stéphanie Flament, Christian Rolando, Richard Ruez, Nathalie Martin, Amélie Decourcelle, Vanessa Dehennaut, Jean-Michel Saliou, Mathias Chamaillard, Corinne Abbadie

published 20 Jan 2023

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SARS-CoV-2 genotyping and sequencing following a simple and economical RNA extraction and storage protocol

Sarah Hernandez, Phuong-Vi Nguyen, Taz Azmain, Anne Piantadosi, Jesse J. Waggoner

published 19 Jan 2023

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New insights from short and long reads sequencing to explore cytochrome b variants in Plasmopara viticola populations collected from vineyards and related to resistance to complex III inhibitors

Semcheddine Cherrad, Benjamin Gillet, Julien Dellinger, Lalie Bellaton, Pascale Roux, Catalina Hernandez, Hervé Steva, Lauriane Perrier, Sébastien Vacher, Sandrine Hughes

published 19 Jan 2023

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Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities

Giulian César da Silva Sá, Leidiane Barboza da Silva, Pedro Vitor Vale Bezerra, Melissa Alves Farias da Silva, Cássio Lázaro Silva Inacio, Weslley de Souza Paiva, Virgínia Penéllope Macedo e Silva, Laísa Vilar Cordeiro, Johny Wysllas de Freitas Oliveira, Marcelo Sousa Silva, Edeltrudes de Oliveira Lima, Francisco José Carvalho Moreira, Hugo Alexandre de Oliveira Rocha, Patricia Batista Barra, Maria de Fátima Freire de Melo Ximenes, Adriana Ferreira Uchôa

published 11 Jan 2023

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Validation of the efficacy of air purifiers using molecular techniques

Finja Rausch, Franziska Tanneberger, Ahmed Abd El Wahed, Uwe Truyen

published 09 Jan 2023

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Immunochemical characterisation of styrene maleic acid lipid particles prepared from Mycobacterium tuberculosis plasma membrane

Sudhir Sinha, Shashikant Kumar, Komal Singh, Fareha Umam, Vinita Agrawal, Amita Aggarwal, Barbara Imperiali

published 06 Jan 2023

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Addressing the quality challenge of a human biospecimen biobank through the creation of a quality management system

Marie-Dominique Servais, Florence Galtier, Agathe Nouvel, Sandra Rebuffat, Jonas Laget, Anne Géan, Nicolas Provost, Frédéric Lorcy, Valérie Rigau, Guilhem Couderc, Philippe Géraud, David Nocca, Nicolas Builles, Nathalie De Préville, Anne-Dominique Lajoix

published 30 Dec 2022

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Rapid, effective and low-cost purification of dideoxy-sequencing reactions by home-made magnetic beads suspension and magnetic separator

Hidenori Sassa, Kota Ikebe

published 22 Dec 2022

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