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Extraction techniques

Molecular characterization of HAMP rs10421768 gene and phenotypic expression of hepcidin; a case-control study among sickle cell anaemia patients in Ghana

Samuel Kwasi Appiah, Charles Nkansah, Gabriel Abbam, Felix Osei-Boakye, Kofi Mensah, Simon Bannison Bani, Solomon Chemogo, Lydia Sarpong, Takyi Godfred Addae, Daniel Boamah Sefa, Richard Adu Croffien, Larry Adom, Rekhiatu Oboirien Abdul Rauf, Farrid Boadu, Godfred Appiah Amoah, Ejike Felix Chukwurah

published 27 Jun 2024

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Comparison of DeNovix, NanoDrop and Qubit for DNA quantification and impurity detection of bacterial DNA extracts

Nick Versmessen, Leen Van Simaey, Abel Abera Negash, Marjolein Vandekerckhove, Paco Hulpiau, Mario Vaneechoutte, Piet Cools

published 17 Jun 2024

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A reverse transcription loop-mediated isothermal amplification assay for quick detection of tomato mosaic virus

Phostine M. Kirasi, Elijah M. Ateka, Edith K. Avedi, Hillary K. Yegon, Bramwel W. Wanjala, Hanu R. Pappu

published 13 Jun 2024

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Exploring the potential of Oxford Nanopore Technologies sequencing for Mycobacterium tuberculosis sequencing: An assessment of R10 flowcells and V14 chemistry

Anzaan Dippenaar, Emilyn Costa Conceição, Felicia Wells, Johannes Loubser, Brendon Mann, Miguel De Diego Fuertes, Vincent Rennie, Robin Mark Warren, Annelies Van Rie

published 06 Jun 2024

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qPCR assay for detection of Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Elements from CAR-T and TCR-T cells in fresh and formalin-fixed tissue

Shalini Pullarkat, Graeme Black, Marie Bleakley, Denise Buenrostro, Aude G. Chapuis, Alexandre V. Hirayama, Carla A. Jaeger-Ruckstuhl, Erik L. Kimble, Bo M. Lee, David G. Maloney, Jerald Radich, Brandon W. Seaton, Jennifer M. Specht, Cameron J. Turtle, David W. Woolston, Jocelyn H. Wright, Cecilia C. S. Yeung

published 06 Jun 2024

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Establishment of dual reverse transcriptase-polymerase chain reaction for detection system for Areca palm velarivirus 1

Chunlin Peng, Benyi Fan, Hui Zhu, Liyun Liu, Zhengwu Zhao, Liyun Huang

published 05 Jun 2024

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Evaluation of short-term hair follicle storage conditions for maintenance of RNA integrity

Eilís E. Harkin, John A. Browne, Barbara A. Murphy

published 31 May 2024

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Description of a ‘plankton filtration bias’ in sequencing-based bacterial community analysis and of an Arduino microcontroller-based flowmeter device that can help to resolve it

Corentin Fournier, Alexander Fiedler, Maximilian Weidele, Harald Kautz, David Schleheck

published 28 May 2024

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Efficient wastewater sample filtration improves the detection of SARS-CoV-2 variants: An extensive analysis based on sequencing parameters

Angelo Robotto, Carlotta Olivero, Elisa Pozzi, Claudia Strumia, Camilla Crasà, Cristina Fedele, Maddalena Derosa, Massimo Di Martino, Stefania Latino, Giada Scorza, Andrea Civra, David Lembo, Paola Quaglino, Enrico Brizio, Denis Polato

published 24 May 2024

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The monsoon-associated equine South African pointy mosquito ‘Aedes caballus’; the first comprehensive record from southeastern Iran with a description of ecological, morphological, and molecular aspects

Jalil Nejati, Shahyad Azari-Hamidian, Mohammad Ali Oshaghi, Hassan Vatandoost, Vanessa L. White, Seyed H. Moosa-Kazemi, Rubén Bueno-Marí, Ahmad A. Hanafi-Bojd, Nancy M. Endersby-Harshman, Jason K. Axford, Fateh Karimian, Mona Koosha, Nayyereh Choubdar, Ary A. Hoffmann

published 23 May 2024

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Genetic diversity and distribution of noroviruses among all age groups of patients with diarrhea in Amhara National Regional State, Ethiopia

Dessie Tegegne, Aschalew Gelaw, Dawit Hailu Alemayehu, Tamrayehu Seyoum, Dereje Leta, Getachew Ferede, Andargachew Mulu, Baye Gelaw

published 21 May 2024

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Tissue distribution of Coxiella burnetii and antibody responses in macropods co-grazing with livestock in Queensland, Australia

Anita Tolpinrud, Mythili Tadepalli, John Stenos, Louis Lignereux, Anne-Lise Chaber, Joanne M. Devlin, Charles Caraguel, Mark A. Stevenson

published 21 May 2024

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Preanalytical considerations for clinical assays of circulating human miRNA-451a, miRNA-423-5p and miRNA-199a-3p for diagnostic purposes

Dinesh S. Chandel, Wesley A. Tom, Chao Jiang, Gary Krzyzanowski, Nirmalee Fernando, Appolinaire Olou, M. Rohan Fernando

published 20 May 2024

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