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Extraction techniques

Detection of SARS-CoV-2 and a possible variant in shelter cats

Ogi Okwumabua, Nancy Bradley-Siemens, Catherine Cruz, Lauren Chittick, Melissa Thompson

published 13 Jan 2025

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Backbone extraction through statistical edge filtering: A comparative study

Ali Yassin, Hocine Cherifi, Hamida Seba, Olivier Togni

published 03 Jan 2025

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A drop dispenser for simplifying on-farm detection of foodborne pathogens

Mohsen Ranjbaran, Simerdeep Kaur, Jiangshan Wang, Bibek Raut, Mohit S. Verma

published 31 Dec 2024

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From museum drawer to tree: Historical DNA phylogenomics clarifies the systematics of rare dung beetles (Coleoptera: Scarabaeinae) from museum collections

Fernando Lopes, Nicole Gunter, Conrad P. D. T. Gillett, Giulio Montanaro, Michele Rossini, Federica Losacco, Gimo M. Daniel, Nicolas Straube, Sergei Tarasov

published 31 Dec 2024

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Development and application of decontamination methods for the re-use of laboratory grade plastic pipette tips

Sang Hyuk Lee, William Kastor, Xiao Fu, Vikas Soni, Michael Keidar, Marc Donohue, Steve Wood, Enusha Karunasena

published 31 Dec 2024

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Optimizing RNA extraction methods for high-throughput transcriptome sequencing of formalin-fixed paraffin-embedded cardiac tissue specimens

Nkechi Martina Odogwu, Jin Sung Jang, Sabrina Albertson, Clinton Hagen, Boyd Rasmussen, Oommen Saji, Timothy J. Nelson, the Wanek Program Preclinical Pipeline

published 26 Dec 2024

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Age-related lung changes linked to altered lysosomal protease profile, histology, and ultrastructure

Mohammed Aufy, Mahmoud Abd-Elkareem, Medina Mustafic, Mostafa A. Abdel-Maksoud, Ali Hakamy, Veronika Baresova, Akram A. Alfuraydi, Mahmoud Ashry, Jana Lubec, Ayman S. Amer, Christian R. Studenik, Ahmed M. Hussein, Mohamed H. Kotob

published 20 Dec 2024

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Laboratory validation of a simplified DNA extraction protocol followed by a portable qPCR detection of M. tuberculosis DNA suitable for point of care settings

Tainá dos Santos Soares, Graziele Lima Bello, Ianca Moraes dos Santos Petry, Maria Rita Castilho Nicola, Larissa Vitoria da Silva, Regina Bones Barcellos, Joana Morez Silvestri, Maria Lucia Rossetti, Alexandre Dias Tavares Costa

published 16 Dec 2024

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Assessment of salivary microRNA by RT-qPCR: Facing challenges in data interpretation for clinical diagnosis

Marc Van Der Hofstadt, Anna Cardinal, Morgane Lepeltier, Jérémy Boulestreau, Alimata Ouedraogo, Malik Kahli, Pierre Champigneux, Laurence Molina, Franck Molina, Thi Nhu Ngoc Van

published 10 Dec 2024

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Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method

Saar Van Overfelt, Hans Duyvejonck, Femke Baeke, Riet De Rycke, Maya Merabishvili, Stefan Vermeulen, Piet Cools, Mario Vaneechoutte, Els Van Mechelen

published 03 Dec 2024

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Specific PCR primer designed from genome data for rapid detection of Fusarium oxysporum f. sp. cubense tropical race 4 in the Cavendish banana

Shunsuke Nozawa, Dan Charlie Joy Pangilinan, G. Alvindia Dionisio, Kyoko Watanabe

published 02 Dec 2024

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RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture

Yuki Kawai-Harada, Mehrsa Mardikoraem, Katherine Lauro, Vasudha Nimmagadda, Quynh Tong, Kayla Bello, Daniel Woldring, Masako Harada

published 02 Dec 2024

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Causality research based on phase space reconstruction

Lei Hu, Zhuoma Sunu, Hongke She, Binghuai Fan, Jingru Ma, Chaojiu Da

published 22 Nov 2024

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