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DNA sequencing

Comparison of qPCR protocols for quantification of “Candidatus Saccharibacteria”, belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA

Stella Papaleo, Riccardo Nodari, Lodovico Sterzi, Enza D’Auria, Camilla Cattaneo, Giorgia Bettoni, Clara Bonaiti, Ella Pagliarini, Gianvincenzo Zuccotti, Simona Panelli, Francesco Comandatore

published 26 Dec 2024

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stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing

Jason D. Limberis, Roland J. Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell, Alina Nalyvayko, Zach Howard, Grant Theron, Rouxjeane Venter, John Z. Metcalfe

published 09 Dec 2024

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MetaCONNET: A metagenomic polishing tool for long-read assemblies

Bingru Sun, Jian Guo, Hao Jin, Zijie Jin, Yaping Sun, Yuanchen Mao, Fuli Xie, Yun He, Zhihong Sun, Wei Li, Igor Ivanov, Hui Tian

published 03 Dec 2024

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Rethreading the needle: A novel molecular index of soil health (MISH) using microbial functional genes to predict soil health management

Heather L. Deel, Daniel K. Manter, Jennifer M. Moore

published 02 Dec 2024

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An easy-to-use pipeline to analyze amplicon-based Next Generation Sequencing results of human mitochondrial DNA from degraded samples

Daniel R. Cuesta-Aguirre, Assumpció Malgosa, Cristina Santos

published 21 Nov 2024

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Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

published 14 Nov 2024

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Combining full-length transcriptome sequencing and next generation sequencing to provide insight into the growth superiority of the hybrid grouper (Cromileptes altivelas (♀) × Epinephelus lanceolatus (♂))

Liu Cao, Jun Ma, Yan Lu, Pan Chen, Xingrong Hou, Ning Yang, Hai Huang

published 09 Oct 2024

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A reproducible approach for the use of aptamer libraries for the identification of Aptamarkers for brain amyloid deposition based on plasma analysis

Cathal Meehan, Soizic Lecocq, Gregory Penner

published 27 Aug 2024

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Germline multigene panel testing in acute and chronic pancreatitis

Mitchell L. Ramsey, Brandie Heald, Yevgeniya Gokun, Josie Baker, J. Royce Groce, Samuel Han, Phil A. Hart, Somashekar G. Krishna, Luis F. Lara, Peter J. Lee, Georgios I. Papachristou, Rachel Pearlman, Sarah Poll, Maegan E. Roberts, Peter P. Stanich

published 22 Aug 2024

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Simulation-guided tunable DNA probe design for mismatch tolerant hybridization

Pallavi Bugga, Vishwaratn Asthana, Rebekah Drezek

published 22 Aug 2024

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Validated DNA isolation method ensuring successful long-read sequencing of cattle semen genome

Erwan Denis, Cécile Grohs, Cécile Donnadieu, Carole Iampietro

published 07 Aug 2024

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Gut microbial composition is altered in sarcopenia: A systematic review and meta-analysis of clinical studies

Xiaohong Mai, Shuyi Yang, Qifeng Chen, Kangkang Chen

published 06 Aug 2024

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The polygenic implication of clopidogrel responsiveness: Insights from platelet reactivity analysis and next-generation sequencing

Omar Echeverría, Mariana Angulo-Aguado, Ricardo Vela, Carlos Calderón-Ospina, Katherine Parra, Nora Contreras, Adrien Morel, Rodrigo Cabrera, Carlos Restrepo, Carolina Ramírez-Santana, Oscar Ortega-Recalde, Manuel Eduardo Rojas-Quintana, Luisa Murcia, Cristian Camilo Gaviria-Sabogal, Nattaly Valero, Dora Janeth Fonseca-Mendoza

published 11 Jul 2024

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