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DNA sequencing

Comparison of qPCR protocols for quantification of “Candidatus Saccharibacteria”, belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA

Stella Papaleo, Riccardo Nodari, [ ... ], Francesco Comandatore

stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing

Jason D. Limberis, Roland J. Nagel, [ ... ], John Z. Metcalfe

MetaCONNET: A metagenomic polishing tool for long-read assemblies

Bingru Sun, Jian Guo, [ ... ], Hui Tian

Rethreading the needle: A novel molecular index of soil health (MISH) using microbial functional genes to predict soil health management

Heather L. Deel, Daniel K. Manter, Jennifer M. Moore

An easy-to-use pipeline to analyze amplicon-based Next Generation Sequencing results of human mitochondrial DNA from degraded samples

Daniel R. Cuesta-Aguirre, Assumpció Malgosa, Cristina Santos

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

Combining full-length transcriptome sequencing and next generation sequencing to provide insight into the growth superiority of the hybrid grouper (Cromileptes altivelas (♀) × Epinephelus lanceolatus (♂))

Liu Cao, Jun Ma, [ ... ], Hai Huang

A reproducible approach for the use of aptamer libraries for the identification of Aptamarkers for brain amyloid deposition based on plasma analysis

Cathal Meehan, Soizic Lecocq, Gregory Penner

Germline multigene panel testing in acute and chronic pancreatitis

Mitchell L. Ramsey, Brandie Heald, [ ... ], Peter P. Stanich

Simulation-guided tunable DNA probe design for mismatch tolerant hybridization

Pallavi Bugga, Vishwaratn Asthana, Rebekah Drezek

Validated DNA isolation method ensuring successful long-read sequencing of cattle semen genome

Erwan Denis, Cécile Grohs, Cécile Donnadieu, Carole Iampietro

Gut microbial composition is altered in sarcopenia: A systematic review and meta-analysis of clinical studies

Xiaohong Mai, Shuyi Yang, Qifeng Chen, Kangkang Chen

The polygenic implication of clopidogrel responsiveness: Insights from platelet reactivity analysis and next-generation sequencing

Omar Echeverría, Mariana Angulo-Aguado, [ ... ], Dora Janeth Fonseca-Mendoza

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