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DNA sequencing

Adaptation of PCR-based library preparation for MGI platform for cancer mutation testing in clinical setting

P.A. Shatalov, M.P. Raygorodskaya, A.P. Shinkarkina, A.A. Traspov, A.V. Murzaeva, Y.A. Doroshenko, I.A. Leuhina, V.A. Mileyko, M. V. Ivanov, T.V. Grigoreva, A. A. Lebedeva, E.M. Veselovsky, L.D. Belyaeva, E. V. Belova, A.D. Kaprin, P.V. Shegai

published 04 Jun 2025

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Host-pathogen protein interaction studies: quality control of cDNA libraries using nanopore sequencing

Cécile Schimmich, Mathilde Gondard, Gregory Caignard, José-Carlos Valle-Casuso, Damien Vitour, François Piumi

published 30 May 2025

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Alpha thalassemia/mental retardation X-linked (ATRX) protein expression in human pituitary neuroendocrine tumours and its reported correlation to prognosis and clinical outcomes: A systematic review

Edward Wang, Fabio Rotondo, Michael D. Cusimano

published 29 May 2025

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Characterization of blood microbial population in beef calves with clinical signs of sepsis using 16S rRNA gene sequencing

Giuliano Borriello, Flaminia Valentini, Sara Ferrini, Giorgia Di Muro, Giulia Cagnotti, Elena Grego, Angela Maria Catania, Maria Cristina Stella, Ugo Ala, Patrizia Nebbia, Antonio D’Angelo, Claudio Bellino

published 23 May 2025

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Diagnosis, clinical features, and mortality risk factors in a Chinese cohort with pulmonary mucormycosis

Junjun Wan, Teng Liu, Fang Li, Shaohua Xu

published 16 May 2025

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Identification of insertion sites for the integrative and conjugative element Tn916 in the Bacillus subtilis chromosome

Emily L. Bean, Janet L. Smith, Alan D. Grossman

published 16 May 2025

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A systematic review and meta-analysis of the diagnostic accuracy after preimplantation genetic testing for aneuploidy

Vanessa Bacal, Angela Li, Heather Shapiro, Urvi Rana, Rhonda Zwingerman, Lisa Avery, Alina Palermo, Eleni Philipoppolous, Crystal Chan

published 14 May 2025

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DNA extraction protocols for animal fecal material on blood spot cards

Ann-Katrin Llarena, Thomas H. A. Haverkamp, Wenche Støldal Gulliksen, Kristin Herstad, Arne Holst-Jensen, Eystein Skjerve, Lisbeth Rannem, Sabrina Rodriguez-Campos, Øivind Øines

published 12 May 2025

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High-performance protocol for ultra-short DNA sequencing using Oxford Nanopore Technology (ONT)

Lukas Žemaitis, Rūta Palepšienė, Simonas Juzėnas, Gediminas Alzbutas, Pierre-Yves Burgi, Thomas Heinis, Jérôme Charmet, Silvia Angeloni Suter, Martin Jost, Renaldas Raišutis, Friedrich Simmel, Ignas Galminas

published 29 Apr 2025

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The canine vaginal microbiome during heat and fertility in healthy breeding dogs

Anna Sophia Leps, Eva-Maria Packeiser, Christina Schwens, Benjamin Stoelcker, Semir Doric, Martina Wirkner, Beate Walter, Axel Wehrend, Viktoria Kichmann, Klaus Jung, Sandra Goericke-Pesch

published 28 Apr 2025

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Rapidly obtaining genome sequence of Severe Fever with Thrombocytopenia Syndrome virus directly from clinical serum specimen using long amplicon based nanopore sequencing workflow

Tingting Tian, Yanhan Wen, Liping Gao, Tiezhu Liu, Xiaoxia Huang, Chuan Li, Shanshan Du, Hao Li, Meijun Guo, Jiandong Li, Shiwen Wang, Dexin Li, Aqian Li, Mifang Liang

published 25 Apr 2025

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A method for PCR-free library preparation for sequencing palaeogenomes

Kirstin Henneberger, Axel Barlow, Federica Alberti, Michaela Preick, Silviu Constantin, Doris Döppes, Wilfried Rosendahl, Michael Hofreiter, Johanna L. A. Paijmans

published 19 Mar 2025

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Error-corrected ultradeep next-generation sequencing for detection of clonal haematopoiesis and haematological neoplasms – sensitivity, specificity and accuracy

Melinda L. Tursky, Crisbel M. Artuz, Melissa Rapadas, Gary A. Wittert, Timothy J. Molloy, David D. Ma

published 26 Feb 2025

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