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DNA sequencing

Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling

Ching Jian, Panu Luukkonen, [ ... ], Katri Korpela

Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications

Peter W. Inglis, Marilia de Castro R. Pappas, Lucileide V. Resende, Dario Grattapaglia

DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples

Michele Simbolo, Marisa Gottardi, [ ... ], Aldo Scarpa

Effects of GC Bias in Next-Generation-Sequencing Data on De Novo Genome Assembly

Yen-Chun Chen, Tsunglin Liu, [ ... ], Chi-Chuan Hwang

Taxonomic Classification of Bacterial 16S rRNA Genes Using Short Sequencing Reads: Evaluation of Effective Study Designs

Orna Mizrahi-Man, Emily R. Davenport, Yoav Gilad

Estimates of Genetic Differentiation Measured by F_ST Do Not Necessarily Require Large Sample Sizes When Using Many SNP Markers

Eva-Maria Willing, Christine Dreyer, Cock van Oosterhout

Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing

Ellen Knierim, Barbara Lucke, [ ... ], Dominik Seelow

Comparison and Validation of Some ITS Primer Pairs Useful for Fungal Metabarcoding Studies

Michiel Op De Beeck, Bart Lievens, [ ... ], Jan V. Colpaert

An Integrated Pipeline for de Novo Assembly of Microbial Genomes

Andrew Tritt, Jonathan A. Eisen, Marc T. Facciotti, Aaron E. Darling

Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products

Gregory B. Gloor, Ruben Hummelen, [ ... ], Gregor Reid

An Improved Genotyping by Sequencing (GBS) Approach Offering Increased Versatility and Efficiency of SNP Discovery and Genotyping

Humira Sonah, Maxime Bastien, [ ... ], François Belzile

The Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing

Yun Huang, William A. Pastor, [ ... ], Anjana Rao

16S rRNA gene sequencing and healthy reference ranges for 28 clinically relevant microbial taxa from the human gut microbiome

Daniel E. Almonacid, Laurens Kraal, [ ... ], Zachary S. Apte

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