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DNA sequencing

Single gene targeted nanopore sequencing enables simultaneous identification and antimicrobial resistance detection of sexually transmitted infections

Liqing Zhou, Andrea Lopez Rodas, Luz Marina Llangarí, Natalia Romero Sandoval, Philip Cooper, Syed Tariq Sadiq

published 21 Jan 2022

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Confounds of using the unc-58 selection marker highlights the importance of genotyping co-CRISPR genes

Helena Rawsthorne-Manning, Fernando Calahorro, Patricia G. Izquierdo, Philippe Tardy, Thomas Boulin, Lindy Holden-Dye, Vincent O’Connor, James Dillon

published 18 Jan 2022

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Evaluation of a parasite-density based pooled targeted amplicon deep sequencing (TADS) method for molecular surveillance of Plasmodium falciparum drug resistance genes in Haiti

Swarnali Louha, Camelia Herman, Mansi Gupta, Dhruviben Patel, Julia Kelley, Je-Hoon M. OH, Janani Guru, Jean F. Lemoine, Michelle A. Chang, Udhayakumar Venkatachalam, Eric Rogier, Eldin Talundzic

published 14 Jan 2022

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Longitudinal variability in the urinary microbiota of healthy premenopausal women and the relation to neighboring microbial communities: A pilot study

Lena M. Biehl, Fedja Farowski, Catharina Hilpert, Angela Nowag, Anne Kretzschmar, Nathalie Jazmati, Anastasia Tsakmaklis, Imke Wieters, Yascha Khodamoradi, Hilmar Wisplinghoff, Maria J. G. T. Vehreschild

published 14 Jan 2022

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NGS read classification using AI

Benjamin Voigt, Oliver Fischer, Christian Krumnow, Christian Herta, Piotr Wojciech Dabrowski

published 22 Dec 2021

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Alu element in the RNA binding motif protein, X-linked 2 (RBMX2) gene found to be linked to bipolar disorder

Pia Laine, William J. Rowell, Lars Paulin, Steve Kujawa, Denise Raterman, George Mayhew, Jennifer Wendt, Daniel L. Burgess, Timo Partonen, Tiina Paunio, Petri Auvinen, Jenny M. Ekholm

published 16 Dec 2021

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CRISPR/Cas9-mediated generation of biallelic F0 anemonefish (Amphiprion ocellaris) mutants

Laurie J. Mitchell, Valerio Tettamanti, Justin S. Rhodes, N. Justin Marshall, Karen L. Cheney, Fabio Cortesi

published 15 Dec 2021

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Analysis of the initial lot of the CDC 2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel

Justin S. Lee, Jason M. Goldstein, Jonathan L. Moon, Owen Herzegh, Dennis A. Bagarozzi Jr., M. Steven Oberste, Heather Hughes, Kanwar Bedi, Dorothie Gerard, Brenique Cameron, Christopher Benton, Asiya Chida, Ausaf Ahmad, David J. Petway Jr., Xiaoling Tang, Nicky Sulaiman, Dawit Teklu, Dhwani Batra, Dakota Howard, Mili Sheth, Wendi Kuhnert, Stephanie R. Bialek, Christina L. Hutson, Jan Pohl, Darin S. Carroll

published 15 Dec 2021

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Local occurrence and fast spread of B.1.1.7 lineage: A glimpse into Friuli Venezia Giulia

Catia Mio, Chiara Dal Secco, Stefania Marzinotto, Claudio Bruno, Santa Pimpo, Elena Betto, Martina Bertoni, Corrado Pipan, Emanuela Sozio, Carlo Tascini, Giuseppe Damante, Francesco Curcio

published 14 Dec 2021

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Development of an efficient Sanger sequencing-based assay for detecting SARS-CoV-2 spike mutations

Ho Jae Lim, Min Young Park, Hye Soo Jung, Youngjin Kwon, Inhee Kim, Dong Kwan Kim, Nae Yu, Nackmoon Sung, Sun-Hwa Lee, Jung Eun Park, Yong-Jin Yang

published 14 Dec 2021

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Variable expression of eighteen common housekeeping genes in human non-cancerous kidney biopsies

Philipp Strauss, Håvard Mikkelsen, Jessica Furriol

published 09 Dec 2021

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Gene excavation and expression analysis of CYP and UGT related to the post modifying stage of gypenoside biosynthesis in Gynostemma pentaphyllum (Thunb.) Makino by comprehensive analysis of RNA and proteome sequencing

Yangmei Zhang, Qicong Chen, Yuanheng Huang, Ruiqiang Zhao, Jian Sun, Xidong Yuan, Huiming Xu, Huiyu Liu, Yaosheng Wu

published 07 Dec 2021

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A scalable high-throughput targeted next-generation sequencing assay for comprehensive genomic profiling of solid tumors

Jeffrey M. Conroy, Sarabjot Pabla, Sean T. Glenn, R. J. Seager, Erik Van Roey, Shuang Gao, Blake Burgher, Jonathan Andreas, Vincent Giamo, Melissa Mallon, Yong Hee Lee, Paul DePietro, Mary Nesline, Yirong Wang, Felicia L. Lenzo, Roger Klein, Shengle Zhang

published 02 Dec 2021

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