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DNA extraction

In silico assessment of 18S rDNA metabarcoding markers for the characterization of nematode communities

Gentile Francesco Ficetola, Alessia Guerrieri, Isabel Cantera, Aurelie Bonin

published 05 Apr 2024

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High-throughput DNA extraction and cost-effective miniaturized metagenome and amplicon library preparation of soil samples for DNA sequencing

Thomas Bygh Nymann Jensen, Sebastian Mølvang Dall, Simon Knutsson, Søren Michael Karst, Mads Albertsen

published 04 Apr 2024

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Genetic identification of three CITES-listed sharks using a paper-based Lab-on-a-Chip (LOC)

Guuske P. Tiktak, Alexandria Gabb, Margarita Brandt, Fernando R. Diz, Karla Bravo-Vásquez, César Peñaherrera-Palma, Jonathan Valdiviezo-Rivera, Aaron Carlisle, Louise M. Melling, Bradley Cain, David Megson, Richard Preziosi, Kirsty J. Shaw

published 04 Apr 2024

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High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method

Lauren Cobb, Erik de Muinck, Spyros Kollias, Morten Skage, Gregor D. Gilfillan, Markus A. K. Sydenham, Shuo-Wang Qiao, Bastiaan Star

published 22 Mar 2024

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Evaluation of a rapid Loop Mediated Isothermal Amplification (LAMP) test for the laboratory diagnosis of sexually transmitted infections

Martina Brandolini, Laura Grumiro, Patrizia Farabegoli, Giorgio Dirani, Silvia Zannoli, Irene Zaghi, Massimiliano Guerra, Francesca Taddei, Giulia Gatti, Anna Marzucco, Maria Sofia Montanari, Alessandra Mistral De Pascali, Simona Semprini, Monica Cricca, Vittorio Sambri

published 21 Mar 2024

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High rate of high-risk human papillomavirus among benign and breast cancer patients in Ethiopia

Esmael Besufikad Belachew, Adey Feleke Desta, Andargachewu Mulu, Dinikisira Bekele Deneke, Dessalegn Abeje Tefera, Ashenafi Alemu, Endale Anberber, Daniel Beshah, Selfu Girma, Dareskedar Tsehay Sewasew, Tesfaye Sisay Tessema, Rawleigh Howe

published 20 Mar 2024

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Detection of pathogenic Leptospira with rapid extraction followed by recombinase polymerase amplification (RPA) and quantitative polymerase chain reaction (qPCR) assay-A comprehensive study from Sri Lanka

Hansi Uduwawala, Aresha Manamperi, Gayana P. S. Gunaratna, Lilani Karunanayake, Arianna Ceruti, Ahmed Abd El Wahed, Lakkumar Fernando, Ranjan Premaratna, Menaka Hapugoda

published 15 Mar 2024

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Field-based molecular detection of Batrachochytrium dendrobatidis in critically endangered Atelopus toads and aquatic habitats in Ecuador

Lenin R. Riascos-Flores, Julio Bonilla, Leopoldo Naranjo-Briceño, Katherine Apunte-Ramos, Grace C. Reyes-Ortega, Marcela Cabrera, José F. Cáceres-Andrade, Andrea Carrera-Gonzalez, Jomira K. Yánez-Galarza, Fausto Siavichay Pesántez, Luis A. Oyagata-Cachimuel, Peter Goethals, Jorge Celi, Christine Van der Heyden, H. Mauricio Ortega-Andrade

published 14 Mar 2024

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Effect of target gene sequence evenness and dominance on real-time PCR quantification of artificial sulfate-reducing microbial communities

Zhe Du, Sebastian F. Behrens

published 07 Mar 2024

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Cross-tissue comparison of telomere length and quality metrics of DNA among individuals aged 8 to 70 years

Sarah E. Wolf, Waylon J. Hastings, Qiaofeng Ye, Laura Etzel, Abner T. Apsley, Christopher Chiaro, Christine C. Heim, Thomas Heller, Jennie G. Noll, Hannah M. C. Schreier, Chad E. Shenk, Idan Shalev

published 22 Feb 2024

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A targeted amplicon next-generation sequencing assay for tryptase genotyping to support personalized therapy in mast cell-related disorders

Olga Li, Jason A. Hackney, David F. Choy, Diana Chang, Rhea Nersesian, Tracy L. Staton, Fang Cai, Shadi Toghi Eshghi

published 09 Feb 2024

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Intracellular presence of Helicobacter pylori antigen and genes within gastric and vaginal Candida

Tingxiu Yang, Jia Li, Yuanyuan Zhang, Zhaohui Deng, Guzhen Cui, Jun Yuan, Jianchao Sun, Xiaojuan Wu, Dengxiong Hua, Song Xiang, Zhenghong Chen

published 08 Feb 2024

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Extraction and selection of high-molecular-weight DNA for long-read sequencing from Chlamydomonas reinhardtii

Frédéric Chaux, Nicolas Agier, Stephan Eberhard, Zhou Xu

published 08 Feb 2024

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