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DNA extraction

Molecular characterization of HAMP rs10421768 gene and phenotypic expression of hepcidin; a case-control study among sickle cell anaemia patients in Ghana

Samuel Kwasi Appiah, Charles Nkansah, Gabriel Abbam, Felix Osei-Boakye, Kofi Mensah, Simon Bannison Bani, Solomon Chemogo, Lydia Sarpong, Takyi Godfred Addae, Daniel Boamah Sefa, Richard Adu Croffien, Larry Adom, Rekhiatu Oboirien Abdul Rauf, Farrid Boadu, Godfred Appiah Amoah, Ejike Felix Chukwurah

published 27 Jun 2024

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Comparison of DeNovix, NanoDrop and Qubit for DNA quantification and impurity detection of bacterial DNA extracts

Nick Versmessen, Leen Van Simaey, Abel Abera Negash, Marjolein Vandekerckhove, Paco Hulpiau, Mario Vaneechoutte, Piet Cools

published 17 Jun 2024

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Exploring the potential of Oxford Nanopore Technologies sequencing for Mycobacterium tuberculosis sequencing: An assessment of R10 flowcells and V14 chemistry

Anzaan Dippenaar, Emilyn Costa Conceição, Felicia Wells, Johannes Loubser, Brendon Mann, Miguel De Diego Fuertes, Vincent Rennie, Robin Mark Warren, Annelies Van Rie

published 06 Jun 2024

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qPCR assay for detection of Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Elements from CAR-T and TCR-T cells in fresh and formalin-fixed tissue

Shalini Pullarkat, Graeme Black, Marie Bleakley, Denise Buenrostro, Aude G. Chapuis, Alexandre V. Hirayama, Carla A. Jaeger-Ruckstuhl, Erik L. Kimble, Bo M. Lee, David G. Maloney, Jerald Radich, Brandon W. Seaton, Jennifer M. Specht, Cameron J. Turtle, David W. Woolston, Jocelyn H. Wright, Cecilia C. S. Yeung

published 06 Jun 2024

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Description of a ‘plankton filtration bias’ in sequencing-based bacterial community analysis and of an Arduino microcontroller-based flowmeter device that can help to resolve it

Corentin Fournier, Alexander Fiedler, Maximilian Weidele, Harald Kautz, David Schleheck

published 28 May 2024

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The monsoon-associated equine South African pointy mosquito ‘Aedes caballus’; the first comprehensive record from southeastern Iran with a description of ecological, morphological, and molecular aspects

Jalil Nejati, Shahyad Azari-Hamidian, Mohammad Ali Oshaghi, Hassan Vatandoost, Vanessa L. White, Seyed H. Moosa-Kazemi, Rubén Bueno-Marí, Ahmad A. Hanafi-Bojd, Nancy M. Endersby-Harshman, Jason K. Axford, Fateh Karimian, Mona Koosha, Nayyereh Choubdar, Ary A. Hoffmann

published 23 May 2024

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Tissue distribution of Coxiella burnetii and antibody responses in macropods co-grazing with livestock in Queensland, Australia

Anita Tolpinrud, Mythili Tadepalli, John Stenos, Louis Lignereux, Anne-Lise Chaber, Joanne M. Devlin, Charles Caraguel, Mark A. Stevenson

published 21 May 2024

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Evaluation of DNAmAge in paired fresh, frozen, and formalin-fixed paraffin-embedded heart tissues

Paulina Pruszkowska-Przybylska, Mikkel Eriksen Dupont, Stine Bøttcher Jacobsen, Morten Smerup, Jacob Tfelt-Hansen, Niels Morling, Jeppe Dyrberg Andersen

published 08 May 2024

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Development, confirmation, and application of a seeded Escherichia coli process control organism to validate Salmonella enterica serovar Typhi environmental surveillance methods

Sarah E. Philo, Nicolette A. Zhou, Lorraine M. Lillis, Venkata Raghava, Dilip Abraham, Vinoth Kumar, Nirmal Kumar, Jonathan Rigby, Joanna Ciol Harrison, Christine S. Fagnant-Sperati, Alexandra L. Kossik, Angelo Q. W. Ong, Rachael Swanstrom, Elisabeth Burnor, Bethel Demeke, Nicola K. Beck, Jeffry H. Shirai, Stephen J. Libby, David S. Boyle, Nicholas Feasey, Gagandeep Kang, John Scott Meschke

published 07 May 2024

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Advancing responsible genomic analyses of ancient mollusc shells

Raphaël Martin-Roy, Jakob Thyrring, Xavier Mata, Pernille Bangsgaard, Ole Bennike, Gunvor Christiansen, Svend Funder, Anne Birgitte Gotfredsen, Kristian Murphy Gregersen, Camilla Haarby Hansen, Peter Carsten Ilsøe, Lutz Klassen, Inge Kjær Kristensen, Gerd Bindesbøl Ravnholt, Frédéric Marin, Clio Der Sarkissian

published 06 May 2024

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OpenCell: A low-cost, open-source, 3-in-1 device for DNA extraction

Aryan Gupta, Justin Yu, Elio J. Challita, Janet Standeven, M. Saad Bhamla

published 02 May 2024

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One-instrument, objective microsatellite instability analysis using high-resolution melt

Kamilla Kolding Bendixen, Sofie Forsberg-Pho, Giulia Dazio, Emeli Elisabeth Hansen, Sarah Kronborg Eriksen, Samantha Epistolio, Elisabetta Merlo, Renzo Boldorini, Tiziana Venesio, Alessandra Movilia, Cecilia Caprera, Eva Christensen Arnspang, Michael Børgesen, Ulf Bech Christensen, Milo Frattini, Rasmus Koefoed Petersen

published 25 Apr 2024

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In silico assessment of 18S rDNA metabarcoding markers for the characterization of nematode communities

Gentile Francesco Ficetola, Alessia Guerrieri, Isabel Cantera, Aurelie Bonin

published 05 Apr 2024

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