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DNA extraction

Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method

Saar Van Overfelt, Hans Duyvejonck, Femke Baeke, Riet De Rycke, Maya Merabishvili, Stefan Vermeulen, Piet Cools, Mario Vaneechoutte, Els Van Mechelen

published 03 Dec 2024

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Specific PCR primer designed from genome data for rapid detection of Fusarium oxysporum f. sp. cubense tropical race 4 in the Cavendish banana

Shunsuke Nozawa, Dan Charlie Joy Pangilinan, G. Alvindia Dionisio, Kyoko Watanabe

published 02 Dec 2024

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RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture

Yuki Kawai-Harada, Mehrsa Mardikoraem, Katherine Lauro, Vasudha Nimmagadda, Quynh Tong, Kayla Bello, Daniel Woldring, Masako Harada

published 02 Dec 2024

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iDNA-ITLM: An interpretable and transferable learning model for identifying DNA methylation

Xia Yu, Cui Yani, Zhichao Wang, Haixia Long, Rao Zeng, Xiling Liu, Bilal Anas, Jia Ren

published 31 Oct 2024

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Banana fruit (Musa sp.) DNA-magnetite nanoparticles: Synthesis, characterization, and biocompatibility assays on normal and cancerous cells

David Arregui-Almeida, Martín Coronel, Karina Analuisa, Carlos Bastidas-Caldes, Santiago Guerrero, Marbel Torres, Andrea Aluisa, Alexis Debut, Werner Brämer-Escamilla, Fernanda Pilaquinga

published 14 Oct 2024

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Comparing the effectiveness of environmental DNA and camera traps for surveying American mink (Neogale vison) in northeastern Indiana

Eleanor L. Di Girolamo, Mark A. Jordan, Geriann Albers, Scott M. Bergeson

published 23 Sep 2024

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Field and DNA-barcode based surveys reveal evidence of rare endemic fishes in the Rufiji River Basin

Jackson L. Saiperaki, Silvia F. Materu, Prisila A. Mkenda, Elly J. Ligate, Cyrus Rumisha

published 19 Sep 2024

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Molecular characterization and antibiotic susceptibility of Shiga toxin- producing Escherichia coli (STEC) isolated from raw milk of dairy bovines in Khyber Pakhtunkhwa, Pakistan

Safir Ullah, Saeed Ul Hassan Khan, Tariq Ali, Muhammad Tariq Zeb, Muhammad Hasnain Riaz, Siraj Khan, Sagar M. Goyal

published 03 Sep 2024

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Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture

Alexander J. Yan, Alaina M. Olson, Kris M. Weigel, Angelique K. Luabeya, Erin Heiniger, Mark Hatherill, Gerard A. Cangelosi, Paul Yager

published 15 Aug 2024

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Effects of storage conditions on the microbiota of fecal samples collected from dairy cattle

Ana S. Jaramillo-Jaramillo, J. T. McClure, Henrik Stryhn, Kapil Tahlan, Javier Sanchez

published 09 Aug 2024

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Drosophila melanogaster as a model arthropod carrier for the amphibian chytrid fungus Batrachochytrium dendrobatidis

Alyssa M. Byer, Kaylie M. Nguyen, Tatum S. Katz, Renwei Chen, Cheryl J. Briggs

published 24 Jul 2024

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Method for isolation of high molecular weight genomic DNA from Botryococcus biomass

Ivette Cornejo-Corona, Devon J. Boland, Timothy P. Devarenne

published 24 Jul 2024

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Quality considerations and major pitfalls for high throughput DNA-based newborn screening for severe combined immunodeficiency and spinal muscular atrophy

Jessica Bzdok, Ludwig Czibere, Siegfried Burggraf, Olfert Landt, Esther M. Maier, Wulf Röschinger, Michael H. Albert, Sebastian Hegert, Nils Janzen, Marc Becker, Jürgen Durner

published 28 Jun 2024

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