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DNA extraction

An SDS-NaOH-based method to isolate genome of recombinant adeno-associated virus vectors for physical titer measurement

Xiangying Zhu, Keying Yang, Jinyan Xie, Xilin Feng, Tao Wu, Mengjun Hu, Haijian Wang, Chenghui Yu, Xiaomin Yu, Farhid Hemmatzadeh, Liqing Zhu, Linhua Zhang

published 03 Apr 2025

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A qPCR assay for the detection of Phytophthora abietivora, an emerging pathogen on fir species cultivated as Christmas trees

Guillaume Charron, Marie-Krystel Gauthier, Hervé van der Heyden, Guillaume J. Bilodeau, Philippe Tanguay

published 02 Apr 2025

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A method for PCR-free library preparation for sequencing palaeogenomes

Kirstin Henneberger, Axel Barlow, Federica Alberti, Michaela Preick, Silviu Constantin, Doris Döppes, Wilfried Rosendahl, Michael Hofreiter, Johanna L. A. Paijmans

published 19 Mar 2025

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Capsicum chinense Jacq. fruit plays an immunomodulatory role in cytokine attenuation and DNA damage protection

Srabonti Saha, Md. Altaf Hossain, Humayra Ferdousi, Jobaier Ibne Deen, Akhlak Chowdhury, Md. Mannan, Md. Asif Nadim Khan, Md. Atiar Rahman

published 25 Feb 2025

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A highly cost-effective, eco-friendly tissue lysis and extraction method for faster DNA isolation from fish fin

Pritam Lenka, Namrata Singh, Deepra Ghosh, Vivekanand Mahato, Sajalendu Ghosh

published 18 Feb 2025

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Exploration of different quantitative polymerase chain reaction-based genotyping methods to distinguish Apc^min/+ mice from wildtype mice

Yuting Sun, Tingyu Zhou, Silin Ye, Effie Yin Tung Lau, Yao Zeng, Jessie Qiaoyi Liang

published 04 Feb 2025

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Nebulization of 2% lidocaine has no detectable impact on the healthy equine respiratory microbiota

Lauren Holley, Hannah N. Creasey, Daniela Bedenice, Sarah Reed, Debora Regina Romualdo da Silva, Victoria Trautwein, Melissa Mazan, Giovanni Widmer

published 24 Jan 2025

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A drop dispenser for simplifying on-farm detection of foodborne pathogens

Mohsen Ranjbaran, Simerdeep Kaur, Jiangshan Wang, Bibek Raut, Mohit S. Verma

published 31 Dec 2024

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From museum drawer to tree: Historical DNA phylogenomics clarifies the systematics of rare dung beetles (Coleoptera: Scarabaeinae) from museum collections

Fernando Lopes, Nicole Gunter, Conrad P. D. T. Gillett, Giulio Montanaro, Michele Rossini, Federica Losacco, Gimo M. Daniel, Nicolas Straube, Sergei Tarasov

published 31 Dec 2024

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Development and application of decontamination methods for the re-use of laboratory grade plastic pipette tips

Sang Hyuk Lee, William Kastor, Xiao Fu, Vikas Soni, Michael Keidar, Marc Donohue, Steve Wood, Enusha Karunasena

published 31 Dec 2024

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Laboratory validation of a simplified DNA extraction protocol followed by a portable qPCR detection of M. tuberculosis DNA suitable for point of care settings

Tainá dos Santos Soares, Graziele Lima Bello, Ianca Moraes dos Santos Petry, Maria Rita Castilho Nicola, Larissa Vitoria da Silva, Regina Bones Barcellos, Joana Morez Silvestri, Maria Lucia Rossetti, Alexandre Dias Tavares Costa

published 16 Dec 2024

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Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method

Saar Van Overfelt, Hans Duyvejonck, Femke Baeke, Riet De Rycke, Maya Merabishvili, Stefan Vermeulen, Piet Cools, Mario Vaneechoutte, Els Van Mechelen

published 03 Dec 2024

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Specific PCR primer designed from genome data for rapid detection of Fusarium oxysporum f. sp. cubense tropical race 4 in the Cavendish banana

Shunsuke Nozawa, Dan Charlie Joy Pangilinan, G. Alvindia Dionisio, Kyoko Watanabe

published 02 Dec 2024

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