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DNA extraction

Nested PCR amplification of the mitochondrial hypervariable region for non-invasive eDNA detection of Cryptobranchus alleganiensis

Patrick Kurtz, Jorge Santo Domingo, Robert Alexander Pyron, David Wendell

published 23 Jul 2025

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SERS-based detection of DNA methylation for cancer diagnosis: Cation-mediated adsorption to silver nanoparticles

Stefania D. Iancu, Vlad Moisoiu, Bogdan Adrian Tigu, Andrei Stefancu, Oana M. Biro, Ciprian Tomuleasa, Nicolae Leopold

published 13 Jun 2025

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Perish the thawed? EDTA reduces DNA degradation during extraction from frozen tissue

Ella Messner, Lev Becker, Mia L. DeSanctis, Elizabeth A. Soranno, Ryan Pianka, Caileigh Pierce, Molly Johnson, Rosalia Falco Poulin, Hannah J. Appiah-Madson, Daniel L. Distel

published 03 Jun 2025

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DNA sequencing of whole human cytomegalovirus genomes from formalin-fixed, paraffin-embedded tissues from congenital cytomegalovirus disease cases

Kathy K. Li, Nicolás M. Suárez, Salvatore Camiolo, Andrew J. Davison, Richard J. Orton

published 30 May 2025

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Making the best of a bad sample: Comparison of DNA extraction and quantification methods using sub-optimally stored Ixodes ricinus ticks

Andrea P. Cotes-Perdomo, Kenedith Méndez-Gutierrez, Kristian Alfsnes, Åshild K. Andreassen, Andrew Jenkins

published 29 May 2025

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Optimization of the viability PCR for accurate detection of Staphylococcus aureus in food samples

Mai Dinh Thanh, Gemma Agustí, Anneluise Mader, Francesc Codony

published 23 May 2025

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A minimally invasive marine mammal sex determination method using epidermal tissue recovered from suction-cup tags

Sadie X. Novak, Jacob M.J. Linsky, Kaitlyn J. Knapp, Susan E. Parks, David Wiley, Dana A. Cusano

published 23 May 2025

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DNA extraction protocols for animal fecal material on blood spot cards

Ann-Katrin Llarena, Thomas H. A. Haverkamp, Wenche Støldal Gulliksen, Kristin Herstad, Arne Holst-Jensen, Eystein Skjerve, Lisbeth Rannem, Sabrina Rodriguez-Campos, Øivind Øines

published 12 May 2025

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Comparative study of Taqman-based qPCR assay for the detection of Anisakis simplex and Pseudoterranova decipiens

Mi-Gyeong Kim, Min Ji Hong, Doo Won Seo, Hyun Mi Jung, Hyun-Ja Han, Seung Hwan Kim, Insun Joo

published 25 Apr 2025

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Implementation of a high throughput automated platform for residual DNA quantitation

Shruti B. Patel, Jon Jurica, Xiaoqing Hua

published 24 Apr 2025

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Colorimetric dual DNAzyme reaction triggered by loop-mediated isothermal amplification for the visual detection of Shiga toxin-producing Escherichia coli in food matrices

Alaa H. Sewid, Joseph H. Ramos, Haley C. Dylewski, Gillian I. Castro, Doris H. D’Souza, Shigetoshi Eda

published 23 Apr 2025

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Molecular detection of Bartonella spp. DNA in dogs with hemangiosarcoma

Cynthia Robveille, Ricardo G. Maggi, Erin Lashnits, Taryn A. Donovan, Keith E. Linder, Daniel P. Regan, Kevin D. Woolard, Edward B. Breitschwerdt

published 22 Apr 2025

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An SDS-NaOH-based method to isolate genome of recombinant adeno-associated virus vectors for physical titer measurement

Xiangying Zhu, Keying Yang, Jinyan Xie, Xilin Feng, Tao Wu, Mengjun Hu, Haijian Wang, Chenghui Yu, Xiaomin Yu, Farhid Hemmatzadeh, Liqing Zhu, Linhua Zhang

published 03 Apr 2025

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