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DNA extraction

Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees

Temitope Ruth Folorunso, Gabriel Silva, Marilis E. Girón, Tess Lindow, Micah Persyn, Lori Eckhardt, Janna R. Willoughby

published 19 Nov 2025

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Rapid on-site universal vertebrate species identification via multi-barcode nanopore sequencing

Emily C. Patterson, Sandrella Morrison-Lanjouw, Mark A. Jobling, Jon H. Wetton

published 11 Nov 2025

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Efficient DNA extraction from cytogenetic suspensions: A new possibility for obtaining DNA, with potential applications in studies of molecular markers

Geórgia Liz Monteiro Sant’ana-Arruda, Paulo Cesar Venere, Daniela Cristina Ferreira

published 07 Nov 2025

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Evidence for an indigenous female mouse urobiome

Sidra Sohail, Daniel Bushnell, Mark Khemmani, Sridhar Narla, Olivia Lamana, Bhanu Sharma, Robert B. Moreland, Alan J. Wolfe, Catherine S. Forster

published 05 Nov 2025

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Evaluation of nanopore sequencing for increasing accessibility of eDNA studies in biodiverse countries

Daniel Gygax, Sabina Ramirez, Moses Chibesa, Twakundine Simpamba, Michael Riffel, Tom Riffel, Amrita Srivathsan, Reindert Nijland, Lara Urban

published 16 Oct 2025

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Microbiome characterization of a pre-Hispanic man from Zimapán, Mexico: Insights into ancient gut microbial communities

Santiago Rosas-Plaza, Luisa Mainou, Gabriela Delgado, Rosario Morales, Ariana Aguilar-Romero, Ana E. Escalante, Rene Cerritos

published 08 Oct 2025

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Microsatellite development for Theridion evexum (Araneae: Theridiidae) using low-coverage genome sequencing and the MiMi script

Ruth Madrigal-Brenes, Gilbert Barrantes, Luis Sandoval, Eric J. Fuchs

published 15 Sep 2025

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Dissecting the dynamics of virus-derived DNA of dengue virus 2 (DENV-2) in Aedes mosquitoes

Hiroka Aonuma, Aboubacar Sombié, Jian-Chiuan Li, Manabu Ote, Erisha Saiki, Hidetoshi Ichimura, Itoe Iizuka, Taichi Odagawa, Tatsuya Sakurai, Kayoko Yamaji, Masayuki Saijo, Chun-Hong Chen, Athanase Badolo, Hirotaka Kanuka

published 12 Sep 2025

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Using eDNA tools to examine the impact of kelp farming on underlying sediments

Samuel H. Tan, Emmaeve Jourdain, Shane P. Farrell, Nichole N. Price, Jeremy J. Rich, David Emerson

published 05 Sep 2025

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Barcode high-resolution melting (Bar-HRM) analysis to authenticate true cinnamon (Cinnamomum verum) from its adulterants and contaminants

M. A. L. M. Peiris, Dhanesha Nanayakkara, Cristian Silva, Sachith P. Abeysundara, Priyanga Wijesinghe

published 02 Sep 2025

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Environmental DNA from peck marks shows potential for non‑invasive monitoring of woodpeckers

Muhammad Bilal Sharif, Björn Ferry, Jérôme Fuchs, Bodil Cronholm, Peter D. Heintzman, Love Dalén

published 20 Aug 2025

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Stable preservation and recovery of methylation marks from FTA elute cards in species with nucleated red blood cells using a customized DNA extraction method

Livia Gerber, Sarah L. Whiteley, Erin E. Hahn, Clare E. Holleley

published 31 Jul 2025

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Methodological establishment and diagnostic value of a multiplex fluorescent PCR assay for the detection of three fastidious respiratory pathogens

Jingchao Shi, Yijun Zhu, Shuyun Chen, Xiaoyun Shan, Lihong Bo, Kai Shen, Keqiang Chen

published 31 Jul 2025

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