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DNA extraction

A DNA metabarcoding approach for recovering plankton communities from archived samples fixed in formalin

Takuhei Shiozaki, Fumihiro Itoh, Yuu Hirose, Jonaotaro Onodera, Akira Kuwata, Naomi Harada

published 17 Feb 2021

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Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

C. H. W. M. R. Bhagya Chandrasekara, D. Nathasha U. Naranpanawa, B. Supun Bandusekara, D. K. N. G. Pushpakumara, D. Siril A. Wijesundera, Pradeepa C. G. Bandaranayake

published 10 Feb 2021

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Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp

Paula Finamore-Araujo, Amanda Faier-Pereira, Carlos Ramon do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy de Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio Cruz Moreira

published 02 Feb 2021

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Testing the effect of pollen exine rupture on metabarcoding with Illumina sequencing

Stephanie J. Swenson, Birgit Gemeinholzer

published 02 Feb 2021

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Detection and differentiation of Burkholderia species with pathogenic potential in environmental soil samples

Sujintana Janesomboon, Veerachat Muangsombut, Varintip Srinon, Chatruthai Meethai, Chayada S. Tharinjaroen, Premjit Amornchai, Patoo Withatanung, Narisara Chantratita, Mark Mayo, Vanaporn Wuthiekanun, Bart J. Currie, Joanne M. Stevens, Sunee Korbsrisate

published 07 Jan 2021

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Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor

Thanawat Sridapan, Wanida Tangkawsakul, Tavan Janvilisri, Wansika Kiatpathomchai, Sirintip Dangtip, Natharin Ngamwongsatit, Duangjai Nacapricha, Puey Ounjai, Surang Chankhamhaengdecha

published 07 Jan 2021

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Assessment of two DNA extraction kits for profiling poultry respiratory microbiota from multiple sample types

Michael E. C. Abundo, John M. Ngunjiri, Kara J. M. Taylor, Hana Ji, Amir Ghorbani, Mahesh K. C., Bonnie P. Weber, Timothy J. Johnson, Chang-Won Lee

published 06 Jan 2021

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Detection of meat from horse, donkey and their hybrids (mule/hinny) by duplex real-time fluorescent PCR

Dan Wang, Liping Wang, Chenyu Xue, Yuebei Han, Hejing Li, Jianqiang Geng, Jiang Jie

published 29 Dec 2020

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Consistent patterns in 16S and 18S microbial diversity from the shells of the common and widespread red-eared slider turtle (Trachemys scripta)

Matthew Parks, Cameron Kedy, Casey Skalla

published 28 Dec 2020

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Influence of sediment and stream transport on detecting a source of environmental DNA

Meredith B. Nevers, Kasia Przybyla-Kelly, Dawn Shively, Charles C. Morris, Joshua Dickey, Murulee N. Byappanahalli

published 28 Dec 2020

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Demonstration of a fast and easy sample-to-answer protocol for tuberculosis screening in point-of-care settings: A proof of concept study

Nasir Ali, Graziele Lima Bello, Maria Lúcia Rosa Rossetti, Marco Aurelio Krieger, Alexandre Dias Tavares Costa

published 14 Dec 2020

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Quantitative PCR assays to detect whales, rockfish, and common murre environmental DNA in marine water samples of the Northeastern Pacific

Elizabeth A. Andruszkiewicz, Kevan M. Yamahara, Collin J. Closek, Alexandria B. Boehm

published 02 Dec 2020

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Development of a sensitive molecular diagnostic assay for detecting Borrelia burgdorferi DNA from the blood of Lyme disease patients by digital PCR

Srirupa Das, Denise Hammond-McKibben, Donna Guralski, Sandra Lobo, Paul N. Fiedler

published 30 Nov 2020

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