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DNA extraction

Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling

Elinor Shvartsman, Meika E. I. Richmond, John J. Schellenberg, Alana Lamont, Catia Perciani, Justen N. H. Russell, Vanessa Poliquin, Adam Burgener, Walter Jaoko, Paul Sandstrom, Kelly S. MacDonald

published 13 Jan 2022

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Fusobacterium nucleatum and Bacteroides fragilis detection in colorectal tumours: Optimal target site and correlation with total bacterial load

Marie S. Rye, Kerryn L. Garrett, Robert A. Holt, Cameron F. Platell, Melanie J. McCoy

published 07 Jan 2022

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Diagnostic accuracy of Truenat Tuberculosis and Rifampicin-Resistance assays in Addis Ababa, Ethiopia

Abyot Meaza, Ephrem Tesfaye, Zemedu Mohamed, Betselot Zerihun, Getachew Seid, Kirubel Eshetu, Miskir Amare, Waganeh Sinshaw, Biniyam Dagne, Hilina Mollalign, Getu Diriba, Melak Getu, Bazezew Yenew, Mengistu Tadesse, Dinka Fikadu, Yeshiwork Abebaw, Shewki Moga, Abebaw Kebede, Habteyes Hailu Tola, Ayinalem Alemu, Muluwork Getahun, Balako Gumi

published 28 Dec 2021

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Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples

K. B. Hoorzook, T. G. Barnard

published 29 Nov 2021

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The effect of soil sample size, for practical DNA extraction, on soil microbial diversity in different taxonomic ranks

Hiroki Morita, Satoshi Akao

published 18 Nov 2021

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Extraction and high-throughput sequencing of oak heartwood DNA: Assessing the feasibility of genome-wide DNA methylation profiling

Federico Rossi, Alessandro Crnjar, Federico Comitani, Rodrigo Feliciano, Leonie Jahn, George Malim, Laura Southgate, Emily Kay, Rebecca Oakey, Richard Buggs, Andy Moir, Logan Kistler, Ana Rodriguez Mateos, Carla Molteni, Reiner Schulz

published 18 Nov 2021

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Development of a real-time PCR assay to detect the single nucleotide polymorphism causing Warmblood Fragile Foal Syndrome

Sharon Flanagan, Áine Rowe, Vivienne Duggan, Erin Markle, Maureen O’Brien, Gerald Barry

published 08 Nov 2021

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The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)

Stephanie S. Coster, Megan N. Dillon, William Moore, George T. Merovich Jr.

published 29 Oct 2021

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How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?

Buddhadeb Roy, Prosenjit Chakraborty, Amalendu Ghosh

published 26 Oct 2021

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Validation study of Boil & Spin Malachite Green Loop Mediated Isothermal Amplification (B&S MG-LAMP) versus microscopy for malaria detection in the Peruvian Amazon

Keare A. Barazorda, Carola J. Salas, Greys Braga, Leonila Ricopa, Julia S. Ampuero, Crystyan Siles, Juan F. Sanchez, Silvia Montano, Stephen E. Lizewski, Christie A. Joya, Danett K. Bishop, Hugo O. Valdivia

published 25 Oct 2021

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Presence of atypical genotypes of Toxoplasma gondii isolated from cats in the state of Bahia, Northeast of Brazil

Ana Graziela de Jesus Deiró, Danielle Paschoal do Prado, Iuri Prates Sousa, Daniele de Santana Rocha, Rodrigo Alves Bezerra, Fernanda Amato Gaiotto, George Rego Albuquerque, Alexandre Dias Munhoz

published 05 Oct 2021

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Multisite verification of the accuracy of a multi-gene next generation sequencing panel for detection of mutations and copy number alterations in solid tumours

John Bartlett, Yutaka Amemiya, Heleen Arts, Jane Bayani, Barry Eng, Daria Grafodatskaya, Suzanne Kamel Reid, Mathieu Lariviere, Bryan Lo, Rebecca McClure, Vinay Mittal, Bekim Sadikovic, Seth Sadis, Arun Seth, Jeff Smith, Xiao Zhang, Harriet Feilotter

published 01 Oct 2021

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Detection of six soil-transmitted helminths in human stool by qPCR- a systematic workflow

Kristy I. Azzopardi, Myra Hardy, Ciara Baker, Rhian Bonnici, Stacey Llewellyn, James S. McCarthy, Rebecca J. Traub, Andrew C. Steer

published 30 Sep 2021

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