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cDNA synthesis

Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming

Thomas P. van Gurp, Lauren M. McIntyre, Koen J. F. Verhoeven

Selection and Validation of Reference Genes for Real-Time Quantitative PCR in Hyperaccumulating Ecotype of Sedum alfredii under Different Heavy Metals Stresses

Jian Sang, Xiaojiao Han, [ ... ], Renying Zhuo

Highly Sensitive Quantitative Real-Time PCR for the Detection of Plasmodium Liver-Stage Parasite Burden following Low-Dose Sporozoite Challenge

Sophie Schussek, Penny L. Groves, Simon H. Apte, Denise L. Doolan

The Origin of Biased Sequence Depth in Sequence-Independent Nucleic Acid Amplification and Optimization for Efficient Massive Parallel Sequencing

Toon Rosseel, Steven Van Borm, [ ... ], Dirk Höper

454 Pyrosequencing to Describe Microbial Eukaryotic Community Composition, Diversity and Relative Abundance: A Test for Marine Haptophytes

Elianne Egge, Lucie Bittner, [ ... ], Bente Edvardsen

In TCR-Stimulated T-cells, N-ras Regulates Specific Genes and Signal Transduction Pathways

Stephen J. Lynch, Jiri Zavadil, Angel Pellicer

Avoiding Pitfalls of Internal Controls: Validation of Reference Genes for Analysis by qRT-PCR and Western Blot throughout Rat Retinal Development

Maurício Rocha-Martins, Brian Njaine, Mariana S. Silveira

Identification of Factors Contributing to Variability in a Blood-Based Gene Expression Test

Michael R. Elashoff, Rachel Nuttall, [ ... ], James A. Wingrove

Comparative Analysis of Gene Expression Level by Quantitative Real-Time PCR Has Limited Application in Objects with Different Morphology

Natalia V. Demidenko, Aleksey A. Penin

Optimizing a qPCR Gene Expression Quantification Assay for S. epidermidis Biofilms: A Comparison between Commercial Kits and a Customized Protocol

Angela França, Ana I. Freitas, Ana F. Henriques, Nuno Cerca

KLC1-ALK: A Novel Fusion in Lung Cancer Identified Using a Formalin-Fixed Paraffin-Embedded Tissue Only

Yuki Togashi, Manabu Soda, [ ... ], Kengo Takeuchi

Highly Parallel Genome-Wide Expression Analysis of Single Mammalian Cells

Jian-Bing Fan, Jing Chen, [ ... ], Jiang F. Zhong

Curcumin-Loaded Apotransferrin Nanoparticles Provide Efficient Cellular Uptake and Effectively Inhibit HIV-1 Replication In Vitro

Upendhar Gandapu, R. K. Chaitanya, [ ... ], Anand K. Kondapi

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