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Biosynthetic techniques

A Novel Pulse-Chase SILAC Strategy Measures Changes in Protein Decay and Synthesis Rates Induced by Perturbation of Proteostasis with an Hsp90 Inhibitor

Ivo Fierro-Monti, Julien Racle, [ ... ], Manfredo Quadroni

Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1

Georgia-Persephoni Voulgaridou, Theodora Mantso, [ ... ], Aglaia Pappa

Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies

Dongmei Hu, Siyi Hu, [ ... ], Jiong Hong

The daytime feeding frequency affects appetite-regulating hormones, amino acids, physical activity, and respiratory quotient, but not energy expenditure, in adult cats fed regimens for 21 days

Alexandra Camara, Adronie Verbrugghe, [ ... ], Anna K. Shoveller

Metabolic Turnover of Synaptic Proteins: Kinetics, Interdependencies and Implications for Synaptic Maintenance

Laurie D. Cohen, Rina Zuchman, [ ... ], Noam E. Ziv

Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway

Hui Yan, Kolapo M. Ajuwon

Protocol Dependence of Sequencing-Based Gene Expression Measurements

Tal Raz, Philipp Kapranov, [ ... ], John F. Thompson

Differences in Muscle Protein Synthesis and Anabolic Signaling in the Postabsorptive State and in Response to Food in 65–80 Year Old Men and Women

Gordon I. Smith, Philip Atherton, [ ... ], Bettina Mittendorfer

A paper-based, cell-free biosensor system for the detection of heavy metals and date rape drugs

Alexander Gräwe, Anna Dreyer, [ ... ], Jörn Kalinowski

Protocol for Nearly Full-Length Sequencing of HIV-1 RNA from Plasma

Yuka Nadai, Lindsay M. Eyzaguirre, [ ... ], Jean K. Carr

Stable C and N isotope analysis of hair suggest undernourishment as a factor in the death of a mummified girl from late 19th century San Francisco, CA

Jelmer W. Eerkens, Bryna Hull, [ ... ], Richard E. Green

Optimizing a qPCR Gene Expression Quantification Assay for S. epidermidis Biofilms: A Comparison between Commercial Kits and a Customized Protocol

Angela França, Ana I. Freitas, Ana F. Henriques, Nuno Cerca

Effects of Arachidonic Acid Supplementation on Acute Anabolic Signaling and Chronic Functional Performance and Body Composition Adaptations

Eduardo O. De Souza, Ryan P. Lowery, [ ... ], Michael D. Roberts

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