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RNA extraction

Assessment of RNA extraction protocols from cladocerans

Muhammad Raznisyafiq Razak, Ahmad Zaharin Aris, Fatimah Md Yusoff, Zetty Norhana Balia Yusof, Sang Don Kim, Kyoung Woong Kim

published 26 Apr 2022

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The challenge of SARS-CoV-2 environmental monitoring in schools using floors and portable HEPA filtration units: Fresh or relic RNA?

Rogelio Zuniga-Montanez, David A. Coil, Jonathan A. Eisen, Randi Pechacek, Roque G. Guerrero, Minji Kim, Karen Shapiro, Heather N. Bischel

published 22 Apr 2022

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A rapid, specific, extraction-less, and cost-effective RT-LAMP test for the detection of SARS-CoV-2 in clinical specimens

Francesco Elia Marino, Eric Proffitt, Eugene Joseph, Arun Manoharan

published 11 Apr 2022

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Performance characteristics of the first Food and Drug Administration (FDA)-cleared digital droplet PCR (ddPCR) assay for BCR::ABL1 monitoring in chronic myelogenous leukemia

Dawne N. Shelton, Prasanthi Bhagavatula, Nathan Sepulveda, Lan Beppu, Shital Gandhi, Dahui Qin, Scott Hauenstein, Jerald Radich

published 17 Mar 2022

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Sequencing SARS-CoV-2 from antigen tests

Ashley Nazario-Toole, Holly M. Nguyen, Hui Xia, Dianne N. Frankel, John W. Kieffer, Thomas F. Gibbons

published 08 Feb 2022

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External quality assessment of COVID-19 real time reverse transcription PCR laboratories in India

Harmanmeet Kaur, Labanya Mukhopadhyay, Nivedita Gupta, Neeraj Aggarwal, Lucky Sangal, Varsha Potdar, Francis Yesuraj Inbanathan, Jitendra Narayan, Swati Gupta, Salaj Rana, Neetu Vijay, Harpreet Singh, Jasmine Kaur, Vinit Kumar, Nirmal Kaundal, Priya Abraham, Vasanthapuram Ravi

published 08 Feb 2022

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Population wide testing pooling strategy for SARS-CoV-2 detection using saliva

Eduardo Esteves, Ana Karina Mendes, Marlene Barros, Cátia Figueiredo, Joana Andrade, Joana Capelo, António Novais, Carla Rebelo, Rita Soares, Ana Nunes, André Ferreira, Joana Lemos, Ana Sofia Duarte, Raquel M. Silva, Liliana Inácio Bernardino, Maria José Correia, Ana Cristina Esteves, Nuno Rosa

published 28 Jan 2022

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Persistence of poliovirus types 2 and 3 in waste-impacted water and sediment

Allison Kline, Kara Dean, Alexandra L. Kossik, Joanna Ciol Harrison, James D. Januch, Nicola K. Beck, Nicolette A. Zhou, Jeffry H. Shirai, David S. Boyle, Jade Mitchell, John Scott Meschke

published 26 Jan 2022

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Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings

Joaquín Moreno-Contreras, Marco A. Espinoza, Carlos Sandoval-Jaime, Marco A. Cantú-Cuevas, Daniel A. Madrid-González, Héctor Barón-Olivares, Oscar D. Ortiz-Orozco, Asunción V. Muñoz-Rangel, Cecilia Guzmán-Rodríguez, Manuel Hernández-de la Cruz, César M. Eroza-Osorio, Carlos F. Arias, Susana López

published 25 Jan 2022

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An international, interlaboratory ring trial confirms the feasibility of an extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples

Margaret G. Mills, Emily Bruce, Meei-Li Huang, Jessica W. Crothers, Ollivier Hyrien, Christopher A. L. Oura, Lemar Blake, Arianne Brown Jordan, Susan Hester, Leah Wehmas, Bernard Mari, Pascal Barby, Caroline Lacoux, Julien Fassy, Pablo Vial, Cecilia Vial, Jose R. W. Martinez, Olusola Olalekan Oladipo, Bitrus Inuwa, Ismaila Shittu, Clement A. Meseko, Roger Chammas, Carlos Ferreira Santos, Thiago José Dionísio, Thais Francini Garbieri, Viviane Aparecida Parisi, Maria Cassia Mendes-Correa, Anderson V. de Paula, Camila M. Romano, Luiz Gustavo Bentim Góes, Paola Minoprio, Angelica C. Campos, Marielton P. Cunha, Ana Paula P. Vilela, Tonney Nyirenda, Rajhab Sawasawa Mkakosya, Adamson S. Muula, Rebekah E. Dumm, Rebecca M. Harris, Constance A. Mitchell, Syril Pettit, Jason Botten, Keith R. Jerome

published 13 Jan 2022

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A method for campus-wide SARS-CoV-2 surveillance at a large public university

Terren Chang, Jolene M. Draper, Anouk Van den Bout, Ellen Kephart, Hannah Maul-Newby, Yvonne Vasquez, Jason Woodbury, Savanna Randi, Martina Pedersen, Maeve Nave, Scott La, Natalie Gallagher, Molly M. McCabe, Namrita Dhillon, Isabel Bjork, Michael Luttrell, Frank Dang, John B. MacMillan, Ralph Green, Elizabeth Miller, Auston M. Kilpatrick, Olena Vaske, Michael D. Stone, Jeremy R. Sanford

published 17 Dec 2021

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Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study

Masaki Karino, Mizuki Harada, Chihiro Yamada, Kyoko Fukuoka, Megumi Sugo, Hiroyuki Hanada, Daisaku Masuda, Shingo Adachi, Shota Nakao, Masayuki Seki, Masaya Yamato, Shizuya Yamashita, Toru Takano

published 14 Dec 2021

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Identification of the best housekeeping gene for RT-qPCR analysis of human pancreatic organoids

Alessandro Cherubini, Francesco Rusconi, Lorenza Lazzari

published 08 Dec 2021

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