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Correction: Identification of an optimal exogenous gene insertion site (P–M) and establishment of a reverse genetics system for aMPV/A

  • Yu Guo,
  • Ting Xue,
  • Bingqing Lu,
  • Jiqing Zheng,
  • Yirong Wang,
  • Xueqiao Xie,
  • Huiting Wang,
  • Furui Cao,
  • Yijun Zhang,
  • Jianhua Wang

In Fig 4, the labels on the right side of A, “raMPV/B-PM-EGFP P5, 10, 15, 20”, have been changed to “raMPV/A-PM-EGFP P5, 10, 15, 20” and the label “raMPV-C-HA” in B has been changed to “raMPV/A-EGFP”. Please see the correct Fig 4 here.

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Fig 4. Expression of EGFP in different generations of aMPV/A-PM-EGFP recombinant viruses.

Genetic stability analysis of raMPV/A-PM-EGFP (the optimal recombinant strain) after serial passage (P5, P10, P15, P20; passage 5, 10, 15, 20) in Vero cells, with parental aMPV/C as the negative control and chicken β-actin as the internal reference protein. A Indirect immunofluorescence assay (IFA) for the detection of aMPV-F protein expression in raMPV/A-PM-EGFP at different passages. Scale bar = 100 μm. B Western blotting (WB) analysis of aMPV-F protein and EGFP expression in serially passaged raMPV/A-PM-EGFP. Primary antibodies included a monoclonal antibody against aMPV-F, a specific antibody against EGFP, and a monoclonal antibody against chicken β-actin. Lanes are as follows: 1 = parental aMPV/C (negative control); 2 = raMPV/A-PM-EGFP P5; 3 = raMPV/A-PM-EGFP P10; 4 = raMPV/A-PM-EGFP P15; 5 = raMPV/A-PM-EGFP P20.

https://doi.org/10.1371/journal.pone.0358322.g004

Reference

  1. 1. Guo Y, Xue T, Lu B, Zheng J, Wang Y, Xie X, et al. Identification of an optimal exogenous gene insertion site (P–M) and establishment of a reverse genetics system for aMPV/A. PLoS One. 2026;21(4):e0347597. pmid:42008498