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Correction: The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability

  • Asuka Tanno,
  • Ryutaro Tokutsu,
  • Yoko Arakaki,
  • Noriko Ueki,
  • Jun Minagawa,
  • Kenjiro Yoshimura,
  • Toru Hisabori,
  • Hisayoshi Nozaki,
  • Ken-ichi Wakabayashi

After this article [1] was published, the corresponding author notified PLOS of errors in Fig 5, S1 Fig, and S3 Fig. Specifically:

  • In Fig 5E, the 0, 15, and 30 min control panels are duplicates of the 0, 15, and 30 min +DCMU panels respectively.
  • The legend for S1 Fig shows an incorrect sample number for the swimming velocity.
  • The C. reinhardtii Cell Model +Ca2+ traces presented in S3 Fig were created from data published in Fig 1B of a previous article by the same group [2].

The corresponding author stated that the 0, 15, and 30 min time point panels for the control conditions in Fig 5E are incorrect and provided an updated Fig 5 with the correct 0, 15, and 30 min control panels from the original experiments. The original images underlying all panels in Fig 5 are provided in [3].

They further stated that the C. reinhardtii Cell Model +Ca2+ traces presented in S3 Fig had been inadvertently included in this article and are mirror images of the wild-type data in Fig 1B of [2]. They stated that the C. reinhardtii waveform traces were provided as a comparative example to illustrate the Ca2+-dependent waveform conversion observed in Tetrabaena and were not used for quantitative analyses. A corrected legend for S3 Fig has been provided to correctly acknowledge [2] as the source of this data. A corrected legend for S1 Fig has been provided to correct the sample sizes to n = 20 for beat frequency and n = 15 for swimming velocity.

The raw data underlying all figures are missing from the list of Supporting Information. The authors have provided this data in [3]. With this correction, all relevant data are now provided.

The Data Availability statement in [1] is incorrect. The correct Data Availability statement is: All relevant data are available from https://zenodo.org/records/21438015.

The C. reinhardtii Cell Model +Ca2+ traces presented in S3 Fig report material from [2], published in 1997 by Wiley-Liss, which are not offered under a CC BY license and are therefore excluded from this article’s [1] license.

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Fig 5. Tetrabaena socialis ISA-2-2 showed slow photoaccumulation toward red light in a photosynthesis-dependent manner.

(A) Suspensions of wild-type C. reinhardtii and T. socialis NIES-571 and ISA2−2 strains were illuminated with red light (λ = 640 nm, 30 μmol photons m-2 s-1) from the right side for 15 min. (B) Polar histograms representing the percentage of cells/colonies moving in a particular direction relative to the red light illumination (12 bins of 30°; n = 30 cells/colonies per strain) for 3 s following 30-s illumination. (C) Phototactic indices (average of cosθ) and (D) parallel indices (average of |cosθ|) calculated from (B). Values were not significantly different from random swimming (p > 0.1; Student’s t-test; see Fig 2C and 2D for details). (E, F) Time-lapse observation of T. socialis ISA2−2 photoaccumulation with (E) DCMU (or ethanol as a control) or (F) cycloheximide (CHX) (or DMSO as a control) treatments for 15 min in the dark. After the red light illumination, images of the Petri dishes were captured every 15 min.

https://doi.org/10.1371/journal.pone.0355515.g005

Supporting information

S1 Fig. Motility parameters of T. socialis.

Ciliary beating frequency (left) and swimming velocity of T. socialis NIES-571 (n = 20 for beat frequency and n = 15 for swimming velocity). Bars represent the average values.

https://doi.org/10.1371/journal.pone.0355515.s001

(TIF)

S3 Fig. The ciliary beating waveforms of live C. reinhardtii and T. socialis and of demembranated T. socialis cell models reactivated with 1 mM ATP and 10−3 M Ca2+ were recorded using a high-speed camera and traced.

The +Ca2+ waveform traces of the C. reinhardtii cell model were prepared from data used in images previously published in Fig 1B of Wakabayashi et al. (1997) [2], in which isolated demembranated cilia were reactivated with 20 μM ATP and 10−4 M Ca2+. Ten waveforms per beat were traced.

https://doi.org/10.1371/journal.pone.0355515.s002

(TIF)

References

  1. 1. Tanno A, Tokutsu R, Arakaki Y, Ueki N, Minagawa J, Yoshimura K, et al. The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability. PLoS One. 2021;16(10):e0259138. pmid:34699573
  2. 2. Wakabayashi K, Yagi T, Kamiya R. Ca2+-dependent waveform conversion in the flagellar axoneme of Chlamydomonas mutants lacking the central-pair/radial spoke system. Cell Motil Cytoskeleton. 1997;38(1):22–8. pmid:9295138
  3. 3. Underlying raw data for “The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability. https://zenodo.org/records/21438015