Figures
After this article [1] was published, the corresponding author notified PLOS of errors in Fig 5, S1 Fig, and S3 Fig. Specifically:
- In Fig 5E, the 0, 15, and 30 min control panels are duplicates of the 0, 15, and 30 min +DCMU panels respectively.
- The legend for S1 Fig shows an incorrect sample number for the swimming velocity.
- The C. reinhardtii Cell Model +Ca2+ traces presented in S3 Fig were created from data published in Fig 1B of a previous article by the same group [2].
The corresponding author stated that the 0, 15, and 30 min time point panels for the control conditions in Fig 5E are incorrect and provided an updated Fig 5 with the correct 0, 15, and 30 min control panels from the original experiments. The original images underlying all panels in Fig 5 are provided in [3].
They further stated that the C. reinhardtii Cell Model +Ca2+ traces presented in S3 Fig had been inadvertently included in this article and are mirror images of the wild-type data in Fig 1B of [2]. They stated that the C. reinhardtii waveform traces were provided as a comparative example to illustrate the Ca2+-dependent waveform conversion observed in Tetrabaena and were not used for quantitative analyses. A corrected legend for S3 Fig has been provided to correctly acknowledge [2] as the source of this data. A corrected legend for S1 Fig has been provided to correct the sample sizes to n = 20 for beat frequency and n = 15 for swimming velocity.
The raw data underlying all figures are missing from the list of Supporting Information. The authors have provided this data in [3]. With this correction, all relevant data are now provided.
The Data Availability statement in [1] is incorrect. The correct Data Availability statement is: All relevant data are available from https://zenodo.org/records/21438015.
The C. reinhardtii Cell Model +Ca2+ traces presented in S3 Fig report material from [2], published in 1997 by Wiley-Liss, which are not offered under a CC BY license and are therefore excluded from this article’s [1] license.
(A) Suspensions of wild-type C. reinhardtii and T. socialis NIES-571 and ISA2−2 strains were illuminated with red light (λ = 640 nm, 30 μmol photons m-2 s-1) from the right side for 15 min. (B) Polar histograms representing the percentage of cells/colonies moving in a particular direction relative to the red light illumination (12 bins of 30°; n = 30 cells/colonies per strain) for 3 s following 30-s illumination. (C) Phototactic indices (average of cosθ) and (D) parallel indices (average of |cosθ|) calculated from (B). Values were not significantly different from random swimming (p > 0.1; Student’s t-test; see Fig 2C and 2D for details). (E, F) Time-lapse observation of T. socialis ISA2−2 photoaccumulation with (E) DCMU (or ethanol as a control) or (F) cycloheximide (CHX) (or DMSO as a control) treatments for 15 min in the dark. After the red light illumination, images of the Petri dishes were captured every 15 min.
Supporting information
S1 Fig. Motility parameters of T. socialis.
Ciliary beating frequency (left) and swimming velocity of T. socialis NIES-571 (n = 20 for beat frequency and n = 15 for swimming velocity). Bars represent the average values.
https://doi.org/10.1371/journal.pone.0355515.s001
(TIF)
S3 Fig. The ciliary beating waveforms of live C. reinhardtii and T. socialis and of demembranated T. socialis cell models reactivated with 1 mM ATP and 10−3 M Ca2+ were recorded using a high-speed camera and traced.
The +Ca2+ waveform traces of the C. reinhardtii cell model were prepared from data used in images previously published in Fig 1B of Wakabayashi et al. (1997) [2], in which isolated demembranated cilia were reactivated with 20 μM ATP and 10−4 M Ca2+. Ten waveforms per beat were traced.
https://doi.org/10.1371/journal.pone.0355515.s002
(TIF)
References
- 1. Tanno A, Tokutsu R, Arakaki Y, Ueki N, Minagawa J, Yoshimura K, et al. The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability. PLoS One. 2021;16(10):e0259138. pmid:34699573
- 2. Wakabayashi K, Yagi T, Kamiya R. Ca2+-dependent waveform conversion in the flagellar axoneme of Chlamydomonas mutants lacking the central-pair/radial spoke system. Cell Motil Cytoskeleton. 1997;38(1):22–8. pmid:9295138
- 3. Underlying raw data for “The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability. https://zenodo.org/records/21438015
Citation: Tanno A, Tokutsu R, Arakaki Y, Ueki N, Minagawa J, Yoshimura K, et al. (2026) Correction: The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability. PLoS One 21(8): e0355515. https://doi.org/10.1371/journal.pone.0355515
Published: August 10, 2026
Copyright: © 2026 Tanno et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.